摘要
α-淀粉酶作为一种十分重要的酶制剂,目前工业上都是以微生物发酵法大规模生产的。从耐高温菌株Geobacillus.POT-5基因组中,用PCR的方法扩增得到该菌的α-淀粉酶基因,此基因被克隆、测序,并在原核Escherichia coli表达系统中进行表达,并对该重组酶的酶学性质进行初步的分析。
α-amylase is a very important enzyme, the mass production ofα-amylase is based on microorganism fermentation in the industry at present. The gene ofα-amylases （amyP） is amplified from the genome of the thermostable strain Geobacillus. POT-5 by PCR. This gene is cloned, sequence analysis and expressed in procaryotic expression system in E.coli. This study also develops a preliminary analysis of enzyme properties about the recombinantα-amylase.
出处
《农产品加工（下）》
2014年第8期33-34,37,共3页
Farm Products Processing
基金
国家自然科学基金资助项目(31070139)
关键词
Α-淀粉酶
原核表达
酶学性质
α-amylase
procaryotic expression
enzymatic characteristic
