慢性乙型肝炎患者HBV-DNA水平与肝纤维化相关指标的检测与相关性分析

The tests and correlation analysis of HBV-DNA and hepatic fibrosis indicators in chronic hepatitis B patients

目的探讨慢性乙型肝炎患者血清HBV-DNA水平与肝纤维化血清指标之间的关联。方法用实时荧光定量PCR法检测HBV-DNA水平、用化学发光法检测肝纤维化指标:透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层粘连蛋白。结果 110例乙型肝炎患者血清HBV-DNA水平(对数值)为5.32±1.37,透明质酸水平为(197.81±85.37)mg/mL,Ⅲ型前胶原水平为(142.66±30.28)μg/mL,Ⅳ型胶原水平为(90.34±20.53)μg/mL,层粘连蛋白水平为(143.96±31.79)μg/mL,均高于对照组(P<0.01)。患者血清HBV-DNA水平与肝纤维化4项血清指标之间的相关性较弱,无统计学意义(P>0.05)。结论慢性乙型肝炎患者肝纤维化4项血清指标上升,但患者血清HBV-DNA水平与肝纤维化血清指标检测值之间没有显著的相关性。

Objective To explore the correlation between serum HBV-DNA content and hepatic fibrosis indicators in hchronic epatitis B patients.Methods Serum HBV-DNA content was determined by using real-time fluorescence quantitative PCR and he-patic fibrosis indicators including hyaluronic acid,laminin,procollagen Ⅲ,and collagen Ⅳ which were determined by using chemilu-minescence.Results The logarithm value of serum HBV-DNA content of the 110 hepatitis B patients was 5.32±1.37.Serum hyal-uronic acid concentration was （197.81 ±85.37）mg/mL;procollagen Ⅲ concentration was （142.66 ±30.28）μg/mL.Collagen Ⅳconcentration was （90.34 ±20.53）μg/mL.Laminin was （143.96 ±31.79 ）μg/mL.All these indicators were significantly higher than the control group （P 〈0.01）.But there were no significant linear correlation between serum HBV-DNA content and the four hepatic fibrosis indicators（P 〉0.05）.Conclusion The hepatic fibrosis indicators increased significantly in hepatitis B patients but there were no significant correlations between serum HBV-DNA content and the hepatic fibrosis indicators.