PEG模拟干旱胁迫下非转基因和转csp2基因油菜种子萌发期抗旱性评价

Evaluation of Drought Resistance of Transgenic and Non- transgenic at Seed Germination Stage under PEG- 6000 Stress

冷激蛋白(cold shock proteins Csps)作为细菌中RNA的分子伴侣,含有原始型的冷休克结构域,具有与核酸结合功能,可以防止RNase对mRNA的降解,也能纠正mRNA的折叠错误,为了寻找作物改良的潜在基因资源,从耐辐射奇球菌(Deinnoccus radiodurans DR1)基因组中克隆出csp2基因,利用农杆菌侵染法转化甘蓝型油菜,通过两代纯化得到T2代转基因油菜种子,以15%聚乙二醇溶液(PEG6000)为渗透介质模拟干旱胁迫条件,对非转基因油菜和转csp2基因油菜种子萌发期抗旱性进行评价.结果显示:PEG胁迫下,转csp2基因油菜的发芽率、发芽势、发芽指数、活力指数,以及胚芽和培根的生长发育程度均高于非转基因油菜.从而表明,csp2基因能够促进植物细胞从逆境伤害中快速恢复.

Csp2,one of RNA chaperon in bacteria,has a prototypical cold shock domain（ CSD）with a poly-nucleotide binding function which prevents mRNA from digestion by RNase and rescues misfolded mRNA. In search of candidate genes for crop improvement,csp2 gene was cloned from the genome of Deinnoccus radiodurans （ DR1 ）. The purificated T2 generation of transgenic rapeseeds was got with the method of Agrobacterium-media-ted transform B. napus. The drought-resistance evaluation of transgenic and non-transgenic nape at seed ger-mination stage was studied by simulated drought stress with 15% PEG-6000 solution. The results showed that the growth and development of transgenic nape germination rate,germination energy,germination index,vitality index and the length of embryonic bud and embryonic root were higher than in non transgenic nape. To show that, csp2 gene under drought stress induced cold shock proteins,through the transcription and the readjustment of mo-lecular chaperone,and improve the ability turn csp2 gene rape of drought resistance.