秀珍菇多糖的超声波协同微波提取工艺及其抗氧化活性研究

Ultrasonic-microwave Synergistic Extraction and Antioxidant Activity of Polysaccharides from Pleurotus geesteranus

在单因素试验的基础上,采用Central Composite Design设计对秀珍菇多糖超声波协同微波提取工艺中的液料比、提取时间和微波功率3因子的最优化组合进行了定量研究,建立并分析了各因子与得率关系的数学模型。同时研究了秀珍菇多糖对DPPH的清除效果。结果表明:最佳的工艺条件为液料比10.5∶1(mL/g)、提取时间123 s和微波功率335 W。经试验验证,在此条件下,得率为26.95%,与理论计算值26.83%基本一致。说明回归模型能较好地预测秀珍菇中多糖的得率。秀珍菇多糖对DPPH自由基和羟基自由基都有一定的清除作用,分别在质量浓度50.34μg/mL^503.42μg/mL和12.08μg/mL^96.66μg/mL的范围内,其清除DPPH自由基和羟基自由基的能力分别为30.54%~51.40%和37.16%~95.87%,且清除效果与多糖质量浓度之间都存在明显的量效关系。

The influences of solvent to material ratio, extraction time and microwave power on the yield of the polysaccharides from Pleurotus geesteranus with the ultrasonic-microwave synergistic extraction technique were studied according to the Central Composite Design experimental design based on the single factor experiments. One mathematical model established and analyzed by response surface methodology （RSM） was adequate to describe the relationships between the studied factors and the response of the polysaccharides exWaction yield. The scavenging effect of the polysaccharides on DPPH and .OH free radical was also studied. Based on the canonical analysis, the optimum extraction conditions were obtained as follows： solvent to material ratio 10.5 ： 1 （mL/g）, extraction time 123 s and microwave power 335 W. Under the optimized extraction conditions, it can be included that the polysaccharides extraction yield is 26.95%, which is close to the estimated value 26.83% attained by using regression model. This model can be used to predict the experiment results well. When the concenlrations of the polysaccharides are 50.34 μ/mL - 503.42μg/mL and 12.08 μg/mL - 96.66 μg/mL, the clearance rates of DPPH and ＂OH free radical are 30.54% - 51.40% and 37.16% - 95.87% individually, and the scavenging activities are correlated with the concentrations of the polysaccharides.