摘要
采用荧光原位杂交技术(fluorescence in situ hybridization,FISH),以萝卜基因组特异序列pURsN作探针,分别与19份菜薹与萝卜附加系回交后代BC4A3d进行杂交,检测回交后代中外源萝卜染色体的存在情况。结果显示:只有1份材料BC4A3d-45没有杂交信号,其他18份材料均得到1个杂交信号,表明除了BC4A3d-45没有附加外源萝卜染色体之外,其余18份材料均含有1条外源萝卜染色体;该结果与分子标记鉴定结果一致。
Nineteen plants from the backcross progeny BC4A3d derived from backcrossing radish additional line with tai-tsai were analyzed by using fluorescence in situ hybridization（FISH）technology with radish genomic probe pURsN to detect the exogenous radish chromosome in the backcross individuals,so as to confirm the added radish chromosome in the backcross progenies.The results showed that except BC4A3d-45 had no additional exogenous radish chromosome,other 18 plants of the backcross progeny were added an alien radish chromosome. This result is consistent with that of the previous molecular marker identification.
出处
《中国蔬菜》
北大核心
2014年第9期27-29,共3页
China Vegetables
基金
国家自然科学基金项目(31071793)
关键词
荧光原位杂交技术
萝卜附加系
染色体
Fluorescent in situ hybridization
Radish addition line
Chromosome
