利用响应面法优化全蚀病生防菌YB-81的发酵条件

Optimization of Fermentaion Conditions of the Biocontrol Strain YB-81 Against Gaeumannomyces gramims Using Response Surface Methodology

为了优化全蚀病生防菌株YB-81的发酵培养基及培养条件,提高其对全蚀病的抑制效果,在单因素试验的基础上,利用响应面法(Response surface methodology)对小麦全蚀病生防菌株YB-81的发酵条件进行优化。生防菌株YB-81的最优发酵条件为:时间36h,温度36℃,初始pH值8.0,转速200 r·min-1,牛肉膏+葡萄糖(1∶1)0.53%,蛋白胨0.99%,氯化钠0.20%。在此培养条件下试验,其菌落总数为18.8亿个·mL-1,较基础培养基提高了72%,对全蚀病菌抑制率达到87.2%,较优化前提高了32.5%。生防菌YB-81的最优发酵培养条件的确定,可为该菌大规模生产应用提供理论基础。

In order to raising the antagonistic effect, the growth conditions and culture medium of biocontrol strain YB-81 was opti- mized based on single factor test. The optimal culture conditions of the biocontrol strain YB-81 were determined for 0.53% beef ex- tract＋glucose （1：1）, 0.99% peptone, 0.20% odium chloride, temperature at 36 %, pH 8.0, speeding at 200 r. min-1, the incubation time of 36 h. The total numbers of colony was 1.88×10^9·mL-1, improving by 72%, and the inhibition rate to Gaeumannomyces gramims was 87.2%, improving by 32.5% with the optimal growth conditions. The optimal growth condition of the biocontrol strain YB-81 was identified, providing a theoretical basis for enlargement of production.