戊四氮致痫幼鼠不同脑区脑损伤研究

A Study of the Brain Damage of Pentylenetetrazol-Induced Epilepsy Young Rats

目的：研究S100B蛋白、碱性髓鞘蛋白（MBP）和神经元特异性烯醇酶（NSE）在戊四氮（PTZ）诱导致痫幼鼠海马、额叶、中脑和血清中的动态改变，评鉴癫痫（EP）造成不同脑区的神经损伤。方法雄性SD幼鼠60只，随机分对照组（10只）和实验组（50只）。实验组腹腔注射（IP）PTZ 50 mg/kg 1次，A组IP生理盐水。根据EP发作分级，0～1级9只视为B组，立即取脑；2级以上发作41只，于EP发作后0 h（C＝10）、6 h（D＝11）、24 h （E＝10）、72 h（F＝10）断头，分别取下海马、中脑和额叶皮质，断头前抽取躯干血。采用ELISA法检测血清和各脑区S100B和MBP ，采用EIA法检测血清和各脑区 NSE值。结果 EP发作后，海马、额叶和血清S100B蛋白、MBP和NSE均明显高于对照组。海马和额叶的NSE峰值出现在中脑NSE之前。各脑区和血清S100B蛋白皆于EP后24 h达高峰，而MBP于EP后72 h达高峰。结论 EP发作可以造成大脑不同脑区神经元细胞、胶质细胞和神经髓鞘的损伤。

Objective By ELISA and EIA evaluated the levels of S100B protein ,myelin basic pro-tein （MBP） and Neuron-specific enolase （NSE） in the serum ,hippocampus ,mesencephalon and frontal cortex in pentylenetetrazol （PTZ） induced epilepsy young rats to study the brain damage after seizure at-tacked . Methods 60 male aged 4~5 weeks young SD rats were divided into control and experimental groups randomly . The control group（A=10） was injected intraperioneally （IP） with the same value of normal saline and the experimental group was IP with PTZ （50 mg/kg） . The experimental group was di-vided into groups with or without seizure again （B= 9） ,group B was sacrificed immediately . Seizure groups were decapitated at 0 h（C= 10） ,6 h（D= 11） ,24 h（E= 10） and 72 h（F= 10） after seizures . Blood samples were collected before scarify . Enzyme-immunoassay（EIA） was used to evaluate the chan-ges of NSE in mesencephalon ,hippocampus ,frontal cortex and serum . And the ELISA was applied to e-valuate the levels of S100B and MBP . Results The levels of S100B ,MBP and NSE in the seizure groups were significant higher than that of control group . The level of NSE in frontal cortex ,striatum and hip-pocampus of the seizure group was increased after seizure ,and the level of NSE in serum and mesencepha-lon got its highest at 6 hours after seizure ,and then decreased . At 24 hours after seizure ,the level of NSE in the hippocampus and serum was still higher then that of the control group （P〈0 .01） . Levels of S100B protein and MBP in serum ,hippocampus ,frontal lobe and mesencephalon were remarkable changes after seizure . Values of S100B protein in different brains and serum got the highest at 24 h after seizure and levels of MBP reached the highest at 72 h after seizure . Conclusion The significant increased levels of S100B protein ,MBP and NSE in the brains and serum of PTZ-induced epilepsy young rats may suggest the damage of neuron cell ,myelin fabric and glial cell .