玻璃体腔注射大麻素HU-211治疗鼠青光眼视神经损伤的研究

Clinical study on intravitreal injection of cannabinoid HU-211 for optic nerve damage in glaucoma rats

目的:评价玻璃体腔内注射大麻素HU-211对大鼠青光眼模型视神经的保护作用,为青光眼视神经损伤治疗提供实验依据。方法:采用电凝巩膜表面静脉法制作大鼠青光眼模型18眼,随机分为3组:A组分别隔日一次玻璃体腔注射1mg/0.1mL大麻素HU-211,B组隔日一次玻璃体腔注射0.1mL生理盐水,C组为高眼压组。随机选6只对侧眼为空白对照组,每日观察眼压变化情况,用药4wk后处死大鼠,视网膜冰冻切片,HE染色通过视网膜神经元的密度变化评估大鼠慢性高眼压模型视网膜神经元的损伤程度。结果:B组的凋亡程度及RGC的损伤程度明显高于A组,差异有统计学意义(P<0.05),B组与C组比较差异无统计学意义(P>0.05)。结论:玻璃体腔注射大麻素(HU-211)对大鼠青光眼模型视神经视网膜有明显的保护作用。

AIM： To evaluate the protective effects of intravitreal injection of cannabinoid HU- 211 for optic nerve in glaucoma rats,providing the experimental evidence in the treatment of glaucoma optic nerve injury.METHODS： Glaucoma rats（ 18 eyes） were modeled by electric coagulation sclera surface vein and randomized into 3 groups,group A received intravitreal injection of1 mg /0. 1mL cannabinoid HU- 211 every other day respectively; group B was given intravitreal injection of0. 1mL water every other day, group C was high intraocular pressure（ IOP） group,6 eyes were randomly selected for blank control group（ group D）. IOP was observed every day. The rats were sacrificed after treatment 4wk,froze retina section,HE stain. The density fluctuation of retinal ganglion cell（ RGC） neurons assessment the optic nerve of rat model with chronic high IOP glaucoma were measured.RESULTS： The apoptosis and damage degree of RGC in group B was obviously higher than that in group A,with statistically significant difference（ P〈0.05）. There was no significant difference in groups B and C（ P〈0.05）.CONCLUSION： Intravitreal injection of cannabinoid HU-211 shows obvious protective effect on optic nerve in glaucom rat models.