细胞角蛋白在牙龈鳞癌颈淋巴结中的表达及临床意义

Cytokeratin expression in cervical lymph nodes of patients with squamous cell carcinoma of the gingival

目的研究细胞角蛋白(CK-AE1/AE3)在牙龈鳞状细胞癌颈淋巴结中的表达及临床意义。方法将17例牙龈鳞癌患者病理报告为阳性的28枚淋巴结及139枚阴性淋巴结重新切片,应用广谱细胞角蛋白单克隆抗体(AE1/AE3)作为免疫标志物,采用免疫组织化学(SP法)检测抗体在淋巴结中的表达。结果 167枚淋巴结中,28枚HE染色阳性的淋巴结CK表达均为阳性;另有11枚HE染色阴性淋巴结CK表达为阳性,其位于Ⅰ区6枚,Ⅱ区5枚,共分布在9例患者中。结论采用CK(AE1/AE3)作为免疫标志物的免疫组化法检测牙龈鳞状细胞癌的淋巴结,较传统HE染色法更为准确,有利于早期发现微转移灶,从而为牙龈癌的治疗及预后判断提供依据。

Objective To study the expression of cytokeratin in cervical lymph nodes of patients with squamous cell carcinoma of the glngivaL Methods Immunohistochemical examination with pan-cytokeratin （CK-AE1/AE3） was performed on 28 positive lymph nodes and 139 negative lymph nodes that were from 17 patients with squamous cell carcinoma of gingival. Results 28 lymph nodes with metastasis shown by general pathological examination （HE stain） were positive for CK. 11 lymph nodes（ in 9 patients） that were shown to have no metastasis by general examination were also positive for CK and 6 nodes were found in level Ⅰ and 5 nodes were found in level Ⅱ. Conclusions The immynohistochemieal with pan-eytokeratin （CK-AE1/AE3） is a good method of detecting lymph node micrometastasis in squamous cell carcinoma of the gingival.