双酚A对小鼠囊胚发育和植入的影响

Effects of Bisphenol-A on Blastocyst Development and Implantation

目的 研究双酚A （BPA）对囊胚发育和植入的影响.方法 对妊娠鼠进行完全随机化分组,分为100、300、600 mg/（kg·d）BPA组和对照组,将BPA处理组的孕鼠从妊娠的0.5～3.5 d灌服100、300和600 mg/（kg·d）BPA,着床点染色,统计胚胎的着床数和着床率,检查植入前囊胚的发育和孵化情况,激光扫描共聚焦显微镜观察孵化囊胚的卵裂球分布和含半胱氨酸的天冬氨酸蛋白水解酶3的表达.结果 300 mg/（kg·d）BPA显著降低了孕鼠的着床点数和着床率,而600 mg/（kg·d）BPA处理的孕鼠未观察到着床点,抑制了胚胎的植入.不同浓度的BPA对妊娠第4天囊胚发育率和囊胚总细胞数无影响,但300和600 mg/（kg·d）BPA显著降低了囊胚的孵化率,提高了孵化囊胚中凋亡细胞的数量.结论 高浓度的BPA损伤了附植前囊胚的发育,影响了胚胎的植入.

Objective To determine the effects of bisphenol-A （BPA） on blastocyst development and implantation.Methods According to completely randomized grouping method,90 pregnant mice were divided into 100,300,and 600 mg/（kg · d） BPA groups and control group.BPA-treated pregnant mice were orally administered with BPA at concentrations of 100,300 and 600 mg/（kg · d） from day 0.5 to day 3.5 of their pregnancy.Blastocyst implantation and development were studied.Results In the 300 mg/（kg · d） BPA group,the number of implantation sites and implantation rate were significantly decreased.In the 600 mg/（kg · d） group,no implantation sites were observed among pregnant mice and BPA inhibited embryo implantation.Blastocyst development on day 4 was examined,and findings showed that the development rate and total numbers of blastocysts in BPA treatment groups had no significant difference from the control group.However,BPA at 300 and 600 mg/（kg · d） significantly reduced blastocyst hatching rate and dramatically increased the number of blastocyst apoptotic cells when compared with those in the control group.Conclusion BPA at a high concentration damages the blastocyst development before implantation and inhibits embryo implantation.