安体舒通对糖尿病大鼠肾脏Nephrin蛋白表达的影响

Effect of spironolactone on Protein Expression of Nephrin in kidney of Diabetic Nephropathy Rats

目的探讨安体舒通对糖尿病(DM)大鼠肾脏Nephrin蛋白表达的影响。方法 SD大鼠分为对照组、糖尿病肾病(DN)组、安体舒通组。腹腔注射链脲佐菌素(50 mg/kg)建立糖尿病大鼠模型,实验12周末检测肾功能、蛋白尿、脂蛋白,Western Blot检测足细胞Nephrin蛋白含量(P<0.05)。结果较安体舒通组,DN组大鼠尿素氮(BUN)、血肌酐(Ccr)下降,24 h尿微量白蛋白排泄率(UAER)增多,脂蛋白升高,且肾脏Nephrin蛋白表达下调,差异有显著性意义(P<0.05)。结论安体舒通对糖尿病肾病肾功能有保护作用,其机制可能与其上调Nephrin蛋白有关。

Objective To observe the effects of spironolactone on the expression of nephrin in the kidney of diabetic ne -phrophathy rats.Methods Healthy male Sprague-Dawley（SD）rats were randomly divided into normal control （NC） and experimental groups .Rat models of diabetic nephropathy were established by intraperitoneal injection of streptozotocin ;After the modeling, the experimental group were randomly divided into diabetic nephrophathy （ DN） group and spironolactone group.At the end of 12th week, blood urea nitrogen（BUN）, creatinine clearance rate（Ccr）, 24h urine protein（24hUpro） and lipoprotein A were determined , the expression level of Nephrin protein was observed by western blot .Results Com-pared with spironolactone groups , BUN, Ccr,24Upro and lipoprotein A in DN group statistically increased;the expression level of nephrin protein in spironolactone group was increased compared with DN group .Conclusion Spironolactone can im-prove the renal function of rats with diabetic nephropathy .Its mechanism may be ralited to the increasing of nephrin expres-sion.