视觉神经细胞电生理技术研究进展

Advances of optic nerve cell electrophysiological technique

神经细胞电生理技术作为一种在实践中形成的,具有可操作性的电生理检测方法,可以在同一时间采集到多量神经细胞的动作电位,对于深化研究大脑神经细胞及其网络的工作原理,研发新的神经修复技术有很大的意义。近年来快速发展的神经细胞电生理技术主要有两种类型:膜片钳技术(PCRT)和在体多通道微电极阵列神经信号技术(M-NEMEA)。本文介绍了这两种技术的起源、发展、技术特点,及其在视神经细胞的研究及应用,并对神经细胞电生理技术在视觉中枢研究及应用等方面亟待解决的问题和发展方向进行分析和探讨。

Nerve cell electrophysiological technique formed during practice, as an operable electrophysiological detection method, can collect great potential action of nerve cells at the same time and has great significance of deepening the research on the brain nerve ceils and the working principle of the network, as well as developing new nerve repair technology. In recent years the rapid development of the nerve calls of electrophysiological techniques mainly include two types： patch clamp recording technique （PCRT）and muhichannel microelectrode array neural signals in the body（M-NEMEA）. This paper introduces the origin, development of the two techniques, technical characteristics, and in the research and application of optic nerve cells as well as analysis and discussion on the problems to be solved and the future development direction of nerve cell electrophysiology technology research and application in visual cortex.