山东省潍坊市尖锐湿疣患儿乳头瘤病毒检测和型别分析

Detection and genotyping of human papilloma virus in children with condyloma acuminatum in Weifang city, Shandong province

目的 检测山东省潍坊市尖锐湿疣患儿中人乳头瘤病毒（HPV）感染情况及基因亚型.方法 采用基因扩增技术及导流杂交分型技术,对24例患儿疣体的HPV进行检测分型.结果 24例尖锐湿疣检出23例HPV阳性,阳性率95.8％,20例单一型HPV感染占87.0％,混合感染型3例占13.0％;感染型别依次是HPV6（9/23）,HPV16（6/23）,HPV 11 （5/23）,HPV58（3/23）,HPV18（2/23）,HPV53（1/23）.结论 该地区尖锐湿疣患儿HPV型别以6、16、11、58、18为主,患儿感染HPV型别与生活中密切接触者感染的型别相似.

Objective To detect human papilloma virus （HPV） and to determine its genotype in children with condyloma acuminatum （CA） in Weifang city,Shandong province.Methods Swab specimens were collected from the surface of lesions of 24 children with CA.PCR/flow-through hybridization assay was performed to detect HPV and determine its genotypes in these specimens.Results Among the 24 specimens,95.8% （23/24） were positive for HPV.Of the HPV-positive specimens,87.0% （20/23） harbored single type of HPV,and 13.0% （3/23） multiple types of HPV.The most common genotype was HPV6（9/23）,followed by HPV16 （6/23）,HPV11 （5/23）,HPV58 （3/23）,HPV18 （2/23）,and HPV53 （1/23）.Conclusions HPV 6,16,11,58 and 18 prevail in children patients with CA in Weifang city,Shandong province.Additionally,the HPV genotypes in children patients are similar to those in their close contacts.