透明质酸钠对大鼠脊髓损伤后膀胱黏膜修复的影响

Intravesical infusion of sodium hyaluronate enhances bladder mucosal repairing after spinal cord injury in rats

目的探讨透明质酸钠对大鼠脊髓损伤后膀胱黏膜修复的影响。方法将48只大鼠随机分为正常对照组、模型对照组及透明质酸钠灌注组,每组16只。采用脊髓横断法建立大鼠脊髓损伤的神经源性膀胱模型。通过观察各组大鼠膀胱大体情况、HE染色情况、组织学损伤评分以及膀胱组织中IL-6的表达情况评估其炎症的严重程度。结果正常对照组膀胱组织无明显病理改变;模型对照组膀胱组织可见大量中性粒细胞浸润,黏膜严重充血、出血,上皮细胞坏死,黏膜、黏膜下层及肌层均有炎性水肿;透明质酸钠灌注组膀胱组织可见黏膜充血,少量炎性细胞浸润。模型对照组及透明质酸钠灌注组组织学损伤评分及膀胱组织中IL-6阳性表达率均显著高于正常对照组,透明质酸钠灌注组组织学损伤评分及膀胱组织中IL-6阳性表达率明显低于模型对照组,差异均有统计学意义(P<0 05或0.01)。结论透明质酸钠膀胱灌注可以减轻大鼠脊髓损伤后膀胱黏膜急性炎症反应以及促进损伤后的膀胱黏膜修复。

Objective To investigate the effect of sodium hyaluronate on bladder mucosal repair after spinal cord injury in rats. Methods Forty eight rats were randomly divided into 3 groups with 16 in each. No any treatment for rats in group A （normal control）;neurogenic bladder model of spinal cord injury was induced with spinal cord transection in groups B （model group） and C （treatment group）, then rats in group C was intravesically infused with sodium hyaluronate. The severity of inflammation in blad-der mucosa was evaluated by HE staining, histological injury score and IL- 6 expression. Results Light microscope showed large number of inflammatory cellinfiltration, significant expansion of capil ary, epithelial cellnecrosis in group B;less inflammato-ry cellinfiltration and capil ary dilatation in group C. Histological injury score in group B was significantly higher than that in group C（P〈0.05）. The expression of IL- 6 in group A was significantly lower than that in groups B and C（P〈0.01）;while IL- 6 expression in group C was lower than that in group B（P〈0.05）. Conclusion Intravesical infusion of sodium hyaluronate can relieve acute in-flammation and enhance the repairing of bladder mucosa in rats after spinal cord injury.