hCTR1在非小细胞肺癌中的表达及其临床意义

Expression and clinical significance of hCTR1 in non-small cell lung cancer

目的探讨非小细胞肺癌(NSCLC)组织中hCTR1的表达与患者临床病理特征的关系以及减轻铂类药副反应的临床方法。方法采用免疫组织化学法,检测54例手术后接受含顺铂方案一线化疗的NSCLC患者组织中hCTR1的表达情况,其中35例患者于化疗前后采取水化、还原型谷胱甘肽、维生素B2及姜黄素等措施,分析hCTR1表达与NSCLC患者临床特征、病理类型、分化程度、临床分期、化疗疗效及化疗副反应的关系。结果 hCTR1的免疫阳性反应主要定位于NSCLC细胞的细胞质;hCTR1表达与含顺铂方案的化疗疗效及淋巴结转移呈正相关(P=0.02,P=0.01),而与年龄(P=0.29)、性别(P=0.32)、病理类型(P=0.19)、临床分期(P=0.52)、分化程度(P=0.62)及是否吸烟(P=0.89)无关;化疗副反应大小与性别(P=0.87)及年龄(P=0.47)无关,与在化疗前后是否采取水化、还原型谷胱甘肽、维生素B2及姜黄素等措施有关(P<0.05)。结论 hCTR1的表达可能是NSCLC患者以铂类为基础治疗的化疗疗效及淋巴结转移的重要预测因子。对于有高表达hCTR1的NSCLC患者化疗时,在化疗前后采取水化、还原型谷胱甘肽、维生素B2及姜黄素等措施,可以减轻化疗副反应。

Objective To investigate the expression of human copper transporters 1 （hCTR1） in patients with non-small cell lung cancer （NSCLC） and analyze its correlation with the clinicopathologic features and methods to reduce the clinical side effects of platinum drugs.Methods In the specimens of 54 lung cancer surgical patients treated with first-line cisplatin-based chemotherapy,the expression of hCTR1 protein was determined with immunohistochemistry.Then we analyzed its correlation with NSCLC clinical features,histological type,degree of differentiation,clinical stage,chemotherapy and side effects of chemotherapy.Results The positive immune reaction of hCTR1 was observed mainly in cytoplasmic staining of NSCLC cells.The expression of hCTR1 had positive correlation with the response to cisplatin-based chemotherapy and lymph node metastasis （P =0.02 and 0.01,respectively）.There was no significant correlation between hCTR1 expression and the following factors：age （P=0.29）,sex （P=0.32）,histological type （P=0.19）,clinical stage （P=0.52）,differentiation （P=0.62） and smoking （P =0.89）.The side effects of chemotherapy was not related to age （P =0.47） and sex（P =0.87）.However,hCTR1 expression was significantly related to receiving hydration,reduced glutathione,vitamin B2 and curcumin before and after chemotherapy （P＜0.05）.Conclusior The expression level of hCTR1 is an important factor that predicts the response to platinum-based chemotherapy and lymph node metastasis in NSCLC patients.For NSCLC patients with high hCTR1 expression,receiving hydration,reduced glutathione,vitamin B2 and curcumin before and after chemotherapy may reduce the side effects of chemotherapy.