人绒毛膜促性腺激素诱导JEG-3细胞TGF-β_3的表达

Effect of human chorionic gonadotrophin on TGF-β_3 expression in JEG-3 cell line

目的:探讨人绒毛膜促性腺激素（hCG）对绒癌细胞系表达转化生长因子（TGF-β3）的影响。方法:以绒癌细胞来源的JEG-3细胞系为研究对象,采用半定量逆转录多聚酶链反应（RT-PCR）方法,观察HCG对JEG-3细胞表达TGF-β3 mRNA的影响响。结果:分别用0、50、500、5000、25000 mU/ml hCG处理48h后,JEG-3细胞中TGF-β3 mRNA的含量逐渐被诱导增多,并表现出一定的浓度效应——随hCG作用浓度增高而显著;另外,分别检测受25 000mU/ml hCG处理0、6、12、24、30、36、48h的JEG-3细胞中TGF-β3 mRNA的表达情况,发现TGF-β3表达受hCG的诱导在30h时最强,之后逐渐减弱。结论:hCG可能通过调节TGF-β3在滋养细胞或滋养细胞来源的绒癌细胞中的表达,进而影响细胞的浸润转移。

Objective:To explore whether human chorioic gonadotrophin(hCG) might change the invasiveness of trophoblast by regulating the prodution of TGF-β3. Methods: The effect of hCG on TGF-β3 mRNA expression in JEG-3 cells was investigated by employing the semi-quantiative method of reverse transcription polymerase chain reaction(RT-TCR). Results:TGF-β3 was shown to be expressed in JEG-3 cells. After incubated with 0,50,500,5 000,25 000 mU/ml hCG for 48 hours respectively, the expression of TGF-β3 in JEG-3 cells increased gradually with the concentration of hCG increased. Additionally,TGF-β3 mRNA expression in JEG-3 cells incubated with 25 000 mU/ml hCG experienced a significant induction at 30 h point which was followed by a less significant decrease.Conclusion:The autocrine of TGF-β3 might be involved in the effect of hCG on trophoblast or trophoblast-derived choriocarcinoma cell invasiveness.