窿缘桉树皮化学成分的分离鉴定及其生物活性研究

Isolation,Structural Identification and Bioactivity of Chemical Constituents from the Bark of Eucalyptus exserta F. Muell

采用多种色谱技术从窿缘桉树皮中分离得到3个化合物,经1H NMR、13C NMR和质谱分析分别鉴定为3β-O-反式对羟基肉桂酰基-12-烯-28-齐墩果酸(1)、3β-O-反式对羟基肉桂酰基-2α-羟基-12-烯-28-乌苏酸(2)和3-O-methylellagic acid-4'-O-α-L-rhamnopyranoside(3)。其中,化合物1为首次从桃金娘科植物中分离得到,化合物1、2为首次从桉属植物的树皮中分离得到,化合物1~3均为首次从该植物中分离得到。分别测定分离得到的化合物对福寿螺、南方根结线虫的毒杀活性以及对斜纹夜蛾的卵巢细胞增殖抑制作用。结果表明以上3种化合物对福寿螺有较弱的毒杀活性,在50 mg/L的浓度下,72 h的死亡率分别为20.03%、22.14%和9.84%;在100 mg/L的浓度下,化合物1和2对南方根结线虫表现出较弱的毒杀活性,72 h的死亡率分别为28.12%和42.19%,化合物3没有杀线虫活性;化合物1和2对斜纹夜蛾卵巢细胞的增殖具有明显抑制作用,在20 mg/L浓度下,抑制率分别达到71.07%和84.03%,化合物3对斜纹夜蛾卵巢细胞的活性较弱,在相同测试浓度下,抑制率为44.30%。

Three compounds were isolated from the bark of Eaca/xptus exserta by various column chromatographic tech- niques. Their structures were identified as 3fl-O-traru-coumareyl-12-en- 28-oleanolic acid （1）, 3β-0-trans-coumaroyl- 12-en-28-ursolic acid （2） and 3-O-methylellagie acid-4＇-O-σ-L- rhamnopyranoside （3） by IH NMR,13C NMR and MS analyses. Compound 1 was obtained from the family Myrtaceae for the first time, compounds 1 and 2 were isolated from the bark of Eucalyptus species for the first time,compounds 1 ~ 3 were isolated from E. exserta for the first time. All the isolated compounds were tested for their mollnscicidal activity against Pomacea canalicu^a, nematiciclal activity against Meloidogyne incognita,and growth inhibition effect against Spodoptera litura cells. The results suggested that all com- pounds possessed weak molluacicidal activity against P. canaliculata, with mortalities of 20.03% ,22. 14% and 9.84% after 72 h,respoctively,at a concentration of 50 mg/L. At a concentration of 100 mg/L,compounds 1 and 2 exhibited weak nematicidal activity against M. incogn/ta,with mortalities of 28.12% and 42.19% after 72 h,respectively. Com- pound 3 exhibited no nematieidal activity against M. incognita. Compounds 1 and 2 showed potent cytotoxic activity a- gainst $. litura cells,with inhibition rates of 71.07% and 84.03% ,respectively,at a concentration of 20 mg/L. Com- pound 3 Showed weakly activity against S. l/tufa cells,at the same concentration level,with the irfifibitory rate of 44. 30%.