摘要
采用硅胶色谱柱分离纯化的方法从小槐花药材中分离得到一种黄酮类化合物，根据理化性质及光谱数据鉴定其为异柠檬酚。采用HPLC法对异柠檬酚的含量进行测定，HPLC分析条件如下：色谱柱为Agilent ZOR-BAXSB-C18（250mm×4．6mm，5μm），流动相为甲醇-0．2％磷酸水溶液（70：30），流速为1．0mL／min，检测波长为270nm，柱温为35℃。实验结果表明异柠檬酚在0．011-0．242μg范围与其峰面积呈良好的线性关系；平均回收率为99．76％，RSD为1．42％（n=6），不同采集地的小槐花药材中异柠檬酚的含量存在一定差异。
A flavonoid was isolated from Desmodium caudatum （Thunb.） DC by silica gel column chromatography. The structure of the isolated compound was elucidated on the basis of spectral characteristics and identified as yukovanol. The content of yukovanol in D. caudatum was determined by HPLC. The chromatographic separation were carried out using the following condition：Agilent ZORBAX SB-Cmcolumn （250 mm × 4.6 ram,5 μm） was used with methanol-0. 2% phosphoric acid （70： 30） as mobile phases. The flow rate was 1.0 mL/min. The UV detection wavelength was 270 nm and the column temperature was 35 ℃. The results showed that the linear range of yukovanol were 0. 011-0.242 trg,the yukovanol average recovery as 99.76% ,RSD = 1.42% （n =6）. Distinct differences were found among D. caudatum samples produced in different places.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第9期1450-1453,共4页
Natural Product Research and Development
基金
广西自然科学基金项目(2012GXNSFAA053175)
广西中药质量标准研究重点实验室主任基金项目(201101)
广西科技研究与技术开发计划项目子课题项目(1355001-4-8)
关键词
小槐花
异柠檬酚
分离纯化
HPLC
Desmodium caudatum （Thunb.） DC
yukovanol
isolation and purification
HPLC
