青蒿内生真菌Alternaria sp.(PQH12)提取物体外细胞毒活性及抗氧化活性的研究

Cytotoxic and Antioxidant Activities of Extracts of Endophytic Fungus Alternaria sp.(PQH12) from Artemisia annua

本文分别采用环己烷、正丁醇、乙酸乙酯、丙酮、甲醇5种极性不同的溶剂分别提取青蒿内生真菌Alternaria sp.(PQH12)的菌丝体。采用MTT法分别测定不同极性溶剂提取物对3种肿瘤细胞株(HL-60、MCF-7和COLO205)的生长抑制率,采用DPPH自由基清除法和超氧阴离子O-·2清除法评价了青蒿内生真菌Alternaria sp.(PQH12)不同极性溶剂提取物的抗氧化活性。结果显示,正丁醇和乙酸乙酯提取物均表现出对肿瘤细胞生长的抑制作用;浓度为20μg/mL时,正丁醇提取物对HL-60细胞的生长抑制率为80.56%,乙酸乙酯提物对MCF-7和COLO205细胞的生长抑制率分别为54.47%和83.09%;在两种抗氧化活性评价体系中,甲醇提取物的抗氧化活性均明显优于其他溶剂的提取物,其IC50值分别为0.20 mg/mL(DPPH)和0.19 mg/mL(O-·2)。该结果表明,青蒿内生真菌Alternaria sp.(PQH12)具有较好的细胞毒和抗氧化潜力,具有一定的开发利用价值,可以作为进一步实验研究的对象。

In this study,the endophytic fungus Alternaria sp. PQH12 from Artemisia annua was fermented and its myce- lia were extracted with different solvents including methanol, acetone, ethyl acetate, n-butanol and cyclohexane. Different tumor cell lines （HL-60, MCF-7 and COLO205） were employed to evaluate the eytotoxic activities of the extracts via MTT assay. In addition, the antioxidant activity of the extract was carried out based on the DPPH radical and O2- scaven- ging assays. The results showed that the n-butanol extract （20 μg/mL） was active against HL-60 leukemia cell lines with a growth inhibition of 80.56% ,while the ethyl acetate extract （20μg/mL） was active against MCF-7 breast and COLO205 colon cell lines with a growth inhibition of 54.47% and 83.09% ,respectively. The methanol extract exhibited strong DPPH radical and O2- scavenging activities with IC50 value of 0.20 mg/mL and 0.19 mg/mL respectively. The results suggested that the endophytic fungal Alternaria sp. PQH12 isolated from A. annua exhibited potential cytotoxic and antioxidant activities.