西施舌水提物对链脲佐菌素诱导糖尿病小鼠的降血糖作用(英文)

Hypoglycaemic Effects of Aqueous Extract of Coelomactra antiquata on Streptozotocin-Induced Diabetes Mellitus Mice

采用链脲佐菌素(STZ)诱发糖尿病小鼠模型,观察西施舌水提物对糖尿病小鼠血糖和糖原含量的影响,以及对6-磷酸果糖激酶、丙酮酸激酶、葡萄糖-6-磷酸酶、果糖-1,6-二磷酸酶、糖原合酶、糖原磷酸化酶等糖代谢相关酶活性的影响。研究西施舌对糖尿病小鼠的降血糖效果和机制。结果表明,西施舌水提物能降低正常小鼠血糖水平和提高血糖耐受性,降低糖尿病小鼠的血糖和血液尿素含量,提高血清总蛋白含量。西施舌水提物能调节糖尿病小鼠的肝糖原含量和糖代谢相关酶活性趋于正常水平,其中灌胃西施舌150 mg/kg的糖尿病小鼠调节效果最佳。西施舌水提物可通过调节糖代谢相关酶活性保持糖尿病小鼠血糖稳态。

The effect of aqueous extract of Coelomactra antiquata （AECA） on levels of blood glucose and glycogen, and the activities of glycometabelism enzymes including 6-phosphofructokinase, pyruvate kinase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, glycogen synthesis and glycogen phosphorylase was evaluated using streptozotocin （STZ）- induced diabetes mellitus mice model. The hypoglycemie effect and mechanism of AECA on diabetic mice was investiga- ted. The results revealed that ACEA decreased blood glucose level and increased the tolerance to glucose of normal mice. The levels of blood glucose and blood urea were decreased, and the level of serum proteins were increased in dia- betic mice treated with ACEA as compared with normal diabetic mice. ACEA treatment restored the enzyme activities and glycogen levels to the near-normal range of diabetic mice, in which 150 mg/kg of body weight group showed the best effect. Based on the experimental results, it was deduced that AECA can maintain the glucose homeostasis by control- ling the carbohydrate metabolizing enzymes.