摘要
目的:建立重组猿免疫缺损病毒-人色素上皮衍生因子(simian immunodeficiency virus-human pigment epithelium-derived factor,SIV-hPEDF)注射液中复制型SIV的实时定量PCR检测方法。方法:在提取重组SIV-hPEDF注射液供试品基因组RNA后,采用实时定量PCR的方法,检测供试品中的复制型SIV。结果:经过3次实验,所得TAT RNA标准品标准曲线均线性良好,线性方程为Y=-3.248lgX+38.12,相关系数为0.994;重组SIV-hPEDF注射液供试品中复制型SIV检测结果均为阴性。结论:所建立的复制型SIV实时荧光定量PCR检测方法重复性好、灵敏、准确,可用于重组SIV-hPEDF注射液中复制型SIV的检测。
Objective：To develop a realtime PCR method for the determination of reproducible SIV in recombinant simian immunodeficiency virushuman pigment epitheliumderived factor（ SIVhPEDF） injection. Method：After extraction of the genome RNA in recombinant SIVhPEDF injection,realtime PCR was used to determine reproducible SIV in test samples. Result：The 3 parallel experiments showed the TAT RNA standard curves had good linearity（ r 0. 994）,and the equation was Y =3. 248 lgX ＋ 38. 12. The results were all negative in the determination of reproducible SIV in recombinant SIVhPEDF injection. Conclusion：The established method is repeatable,sensitive and accurate,and applicable in the determination of reproducible SIV in recombinant SIV-hPEDF injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2014年第9期1549-1552,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家863计划项目(No:2012AA020805)
国家科技重大专项项目(No:2012ZX09304010)
