磁性固定化PLA_1的制备及其在脱胶应用中的研究

Preparation of magnetic immobilized PLA_1 and applied in the degumming

本文目的是研究磷脂酶A1的固定化条件并将固定化酶用于脱胶过程。以磁性Fe3O4/SiOx-g-P(GMA)复合粒子为载体,考察不同的固定化条件对固定化酶相对酶活的影响,然后将制得的磁性固定化PLA1用于大豆油脱胶中,并对其酶活再生进行有益研究。结果表明,最优固定化条件为:酶液添加量20mL,pH6.0,反应时间5h,此时酶活力最高;最佳脱胶条件为:反应时间6h、反应温度55℃、加酶量0.12g/kg、油相起始pH6.0。在最优条件下进行脱胶试验,测定脱胶油中的磷含量为12.5mg/kg,脱胶效果较好。将再生的磁性固定化磷脂酶A1再用于大豆油脱胶中,延长了磁性固定化磷脂酶A1的利用时间,降低了大豆油酶法脱胶的生产成本。

This study focused on optimization of immobilization conditions of phospholipase A1, and then the immobilized PLA1 was used in soybean oil degumming process.Phospholipase A1 was immobilized by Fe3O4/SiOx -g - P （ GlVlA）. The obtained magnetic immobilized PLA1 was used for soybean oil degumming, then, the regeneration of magnetic immobilized PLA1 was studied.The results showed that the optimal immobilized conditions were：the enzyme dosage was 20mL, pH was 6.0, the reaction time was 5h, and the relative activity of magnetic immobilized PLA1 was the highest under the optimal conditions.The optimal conditions of degumming were： the reaction time was 6h,the reaction temperature was 55℃,the dosage of magnetic immobilized PLA1 was 0.12g/kg, initial pH of oil phase was 6.0.The residual phosphorus content of degummed soy oil was decreased to 12.5mg/kg under the optimum conditions. The phosphorus could be removed effectively. Then the regenerated magnetic immobilized phospholipase A1 was used in soybean oil degumming,the used time of the magnetic immobilized phospholipase A1 was prolonged,and the cost of enzymatic degumming of soybean oil was reduced.