宽叶金粟兰总黄酮的提取工艺及抗氧化活性研究

Study on extraction process of total flavoniods and its antioxidant effect from Chloranthus henryi

目的：对宽叶金粟兰中总黄酮的提取工艺及抗氧化活性进行了研究，旨在为其开发利用提供理论依据。方法：采用微波辅助提取法对宽叶金粟兰中总黄酮的提取工艺进行优化，采用分光光度法测定提取物的还原力，并研究其对羟基自由基、DPPH自由基的清除作用。结果：最佳提取条件为：乙醇体积分数60％、料液比1：30、微波功率300W，微波处理时间15min；在此条件下宽叶金粟兰的总黄酮提取率为6．11％。在实验浓度（0.2～0．8mg／mL）范围内，宽叶金粟兰黄酮对羟基自由基的清除率可达到100％，对DPPH自由基的清除率达到76．2％，同时还具有较强的还原能力。结论：研究结果表明宽叶金粟兰中的黄酮类物质具有较强的抗氧化活性，该植物具有一定的开发和应用前景。

Objective.To study the extraction technology of total flavonoids from Chloranthus henryi and its antioxidant activity.Methods： The optimum condition for extracting total flavonoids from Chloranthus henryi by microwave- assisted was obtained through orthogonal experiments.And the antioxidant activity of the extract was evaluated by spectrophotometry on its ability of scavenging hydroxyl free radical （. OH）and 1-diphenyl-2-picryJhydrazy free radical（DPPH.） ,total reducing power.Results： The optimum procedure for microwave-assisted extraction of total flavonoids were ethanol concentration of 60%, ratio of material to solvent of 1：30, microwave power of 300W, microwave time of 15min and that the fiavonoids yield was up to 6.11%.The scavenging rates of total flavonoids from Chloranthus henryi against hydroxyl free radical and DPPH free radical were found to be 100% and 76.2%,and the extract also had a strong total reducing power at a certain concentration range （0.2-0.8mg/mL）. Conclusion： The results showed that the extract from Chloranthus henryi was active for oxidation resistance,and it showed good application future for this plant.