PDLIM2基因在膀胱癌组织中的表达及其临床意义

Expression and clinical significance of PDLIM2 in bladder carcinoma

目的 探讨PDLIM2 mRNA在膀胱尿路上皮癌组织与正常膀胱组织中的表达及其临床意义,研究PDLIM2基因在预测膀胱癌术后复发、进展以及预后判断中的价值.方法 应用逆转录-聚合酶链反应（RT-PCR）和实时荧光定量聚合酶链反应（FQ-PCR）技术检测80例膀胱尿路上皮癌组织、10例膀胱黏膜正常组织中PDLIM2 mRNA的表达,并结合临床病理特征进行分析.结果 膀胱癌组织中PDLIM2 mRNA表达量明显低于正常膀胱组织（P＜0.05）,仅是正常组织中表达量的49.8％,其表达水平与膀胱癌的分化程度、病理分期、肿瘤数目、大小相关,随肿瘤分级、分期、数目、直径的增加而减少（P＜0.05）,与性别、年龄无相关（P＞0.05）.单因素分析表明肿瘤分级、分期、数目及PDLIM2 mRNA表达量是膀胱癌患者术后复发或进展的预后因素.Logistic回归分析表明PDLIM2 mRNA表达是评估膀胱癌患者无病生存预后的独立预测因子[相对危险度（RR）=0.982,95％可信区间（CI）=0.967 ～0.997,P＜0.05],PDLIM2表达低的膀胱癌患者较PDLIM2高表达的患者更容易出现复发或进展.多因素分析发现膀胱肿瘤的分级、数目与PDLIM2 mRNA表达明显相关（P＜0.05）.结论 PDLIM2 mRNA的表达与膀胱癌的发生、发展密切相关,可能是预测膀胱癌术后复发或进展的潜在肿瘤标志物.

Objective To investigate the expression of PDLIM2 mRNA in bladder urothelium carcinoma and clinical significance.To explore the values of PDLIM2 in the prediction of postoperative recurrence or progress and prognosis.Methods PDLIM2 mRNA was detected in 80 bladder cancer tissues and 10 normal bladder epithelium tissues by using reverse transcriptase-polymerase chain reaction （RT-PCR） and real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.Correlations between PDLIM2 and clinicopathological features and prognosis were investigated by statistical analyses.Results A significant down regulation of PDLIM2 mRNA expression was observed in the tumor tissues compared with the corresponding normal bladder tissues （P ＜ 0.05）,the mean expression of PDLIM2 mRNA in the cancer samples was 49.8％.The expression level of PDLIM2 mRNA was negatively correlated with the tumor grade,clinical stage,number and size （P ＜ 0.05）,but not with sex and age （P ＞ 0.05）.Patients with low levels of PDLIM2 mRNA expression had a poor prognosis compared with those with a high expression （P ＜0.05）.Univariate and multivariate logistic regression prognostic analyses revealed that PDLIM2 mRNA was an independent prognostic factor for disease-free survival in bladder cancer.Conclusion PDLIM2 is significant in the progression of bladder cancer and the status of PDLIM2 mRNA expression is a novel prognostic marker for predicting bladder cancer disease-free survival.