RP-HPLC法测定新乡种植红豆杉树皮中的三尖杉宁碱

Determination of Cephalomannine in Chinese Yew Planted in Xinxiang by RP-HPLC

建立一种反向高效液相色谱法定量检测蛹虫草培养基中活性成分腺苷含量和虫草素含量的方法.色谱柱为Eclipse XDB-C18柱（4.6 mm×150 mm，5μm）；流动相：0.02 mol/L KH2PO4∶甲醇（体积比85∶15）溶液；流速：1.0 mL/min；检测波长：260 nm .腺苷的线性测量范围为0.5~100μg/mL（R=0.9999），平均回收率为105.3%，标准品RSD=0.76%（n=5），样品RSD=1.87%（n=5）；虫草素的线性测量范围为0.5~100μg/mL（R=0.9999），平均回收率为104.0%，标准品RSD=0.95%，样品RSD=1.23%.本测定方法线性范围宽、分离度好、具有良好精密度，可定量、准确分析不同蛹虫草培养基中活性成分腺苷和虫草素含量.

A method was established for the quantitative determination of bioactive components,adenosine and cordycepin,in culture medium of Cordyceps militaris by reversed-phase high performance liquid chromatography. The separation was performed on Eclipse XDB-C18 column,The mobile phase was 0.02 mol/L phosphate buffer solution-methanol（volume ratio 85∶15）. The flow rate was 1.0 mL/min,and the detection wavelength was 260 nm. The linear measurement range of adenosine was 0.5~100μg/mL（R=0.999 9）;its average recovery was 105.3%;its standard RSD=0.76%（n=5）;and its sample RSD=1.87%（n=5）. The linear measurement range of cordycepin was 0.5~100 μg/mL（R=0.999 9）;its average recovery was 104.0%;its standard RSD=0.95%（n=5）;and its sample RSD=1.23%（n=5）. The method has the characteristics with a wide linear range,a good degree of separation and a good precision. It can be used for quantitative analysis of adenosine and cordycepin in different culture medium for artificial Cordyceps militaris.