GP73联合AFP及GGT-Ⅱ检测对原发性肝癌的诊断价值

Dignostic value of GP73 combined with AFP,GGT-Ⅱ detection on primary liver cancer

目的探讨联合检测高尔基体糖蛋白73(GP73)、甲胎蛋白(AFP)和γ-谷氨酰转移酶同工酶Ⅱ(GGT-Ⅱ)对原发性肝癌的诊断意义。方法分别采用化学发光免疫分析法、ELISA法和聚丙烯酰胺凝胶电泳法检测105例原发性肝细胞癌、60例肝硬化、72例乙型肝炎、30例肝良性肿瘤、90例HBV携带者和50例健康体检者血清中GP73、AFP和GGT-Ⅱ含量,分析GP73联合AFP、GGT-Ⅱ检测对原发性肝癌的诊断价值。结果原发性肝癌组GP73、AFP含量及GGT-Ⅱ阳性率明显高于良性肝病及正常对照组,差异有统计学意义(P<0.05);单独检测GP73、AFP和GGT-Ⅱ对诊断原发性肝癌的特异性分别为75.2%、84.8%和89.1%,敏感性分别为79.0%、57.1%、62.9%,联合检测GP73、AFP和GGT-Ⅱ可显著提高诊断原发性肝癌的敏感性(97.1%),但特异性有所下降。结论联合检测GP73、AFP和GGT-Ⅱ作为诊断原发性肝癌的重要指标,可显著提高原发性肝癌的诊断率。

Objective To explore the diagnostic value of GP73 combined with AFP,GGT- Ⅱ detection on primary liver cancer. Methods Serum GP73, AFP, GGT- Ⅱ levels of 105 cases of primary liver cancer, 60 cases of liver cirrhosis,72 cases of hepatitis B, 30 cases of benign tumor, 90 cases of HBV carriers and 50 cases of healthy controls were detected by ELISA, chemilunescent immunoassay and SDS-PAGE. Analysed the value of GP73 combined with AFP, GGT- Ⅱ detection on primary liver cancer. Results The levels of GP73,AFP and positive rate of GGT- Ⅱ were significantly higher than benign liver disease and healthy control group,the differences were statistically significant （P〈0.05）. Specificity of GP73, AFP and GGT-Ⅱ in the diagnosis of primary liver cancer was 75.2%, 84.8% and 89.1%, respectively. Sensitivity of GP73, AFP and GGT-Ⅱ in the diagnosis of primary liver cancer was 79.0% ,57.1% and 62.9% ,respectively. The joint detection GP73 ,AFP and GGT- Ⅱ could improve the sensitivity for the diagnosis of primary liver cancer, but the specificity decreased. Conclusion The combined detection of GP73 ,AFP and GGT- Ⅱ is an important index for the diagnosis of primary liver cancer,which can improve the diagnosis rate of primary liver cancer.