DBA/1小鼠与C57BL/6小鼠胶原诱导性关节炎模型的比较研究

Comparative study of the collagen-induced arthritis model between DBA/1 and C57BL/6 mice

目的:对胶原诱导性关节炎(collagen-induced arthritis,CIA)在不同品系小鼠(DBA/1小鼠和C57BL/6小鼠)的发病情况进行比较研究,以期寻找出一种适合制备CIA模型的小鼠品系。方法:用鸡Ⅱ型胶原(typeⅡcollagen,CⅡ)乳剂分别免疫DBA/1小鼠和C57BL/6小鼠诱导CIA,进行关节外观的观察及关节炎临床评分。结果:免疫后第31天,DBA/1小鼠出现关节红肿,关节炎临床评分升高,在第39天达到高峰。随着时间的推移,小鼠关节红肿等急性炎症现象有所减退。用改进方法免疫的C57BL/6小鼠于第21天开始出现关节红肿、临床评分升高现象。结论:在制备CIA模型时建议首选DBA/1小鼠。

Objective：Taking collagen-induced arthritis（CIA） as experimental subject,make comparative study on species to quest for the moderate method of making the CIA mice model. Methods：CIA model was induced by chicken type Ⅱcollagen （CⅡ） in DBA/1 and C57BL/6 mice. The joints of the mice were observed for clinical manifestation and clinical score of swelling. Results：Paw-swelling onset was on day 31 after the first immunization in DBA/1 mice. Clinical score for swelling of the paws reached peak on day 39 after the first immunization. As time went on , the acute inflammation of paws decreased. By using an improved method, paw-swelling onset was on day 21 after the first immunization in C57BL/6 mice. Conclusion：The DBA/1 mice were recommend for CIA animal model.