超高效液相色谱-串联质谱法测定化妆品中23种邻苯二甲酸酯类化合物

Determination of 23 kinds of phthalic ester in cosmetics by UPLC-MS /MS

建立了超高效液相色谱-串联质谱法同时测定化妆品中23种邻苯二甲酸酯类化合物的方法。不同类型化妆品用不同方法提取、净化,采用多反应监测（MRM）模式对23种目标化合物进行定性,同位素内标标准曲线法定量。结果表明,23种邻苯二甲酸酯类化合物的质量浓度在0.01~1.0 mg·L-1内,方法具有良好的线性,相关系数均大于0.998,检出限为0.003~0.095μg·g-1,平均回收率（n=9）为86.1%~100.5%,相对标准偏差为0.74%~3.23%。方法学实验还表明,该方法具有良好的重复性、精密度和稳定性。

A method for determination of 23 kinds of phthalic ester in cosmetics simultaneously by UPLC - MS/ MS was established. Various types of cosmetics were extracted and purified by different methods. MRM mode was used to qualitatively identify the 23 target compounds and isotope internal standards were used to quantify by calibration curves. In the range of 0. 01 - 1.0 mg ·L-1 , the linearity of the method is good and the correlation coefficients are over 0. 998. The LOD of the method ranges within 0. 003 - 0. 095 μg· g-1. Theaverage recovery of the method ranges within 86. 1% - 100. 5% （ n = 9） and the RSD ranges within 0. 74% - 3.23%. Methodology experiment displayed that the reproducibility, precision and stability of the method are good.