超微铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的影响

Effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal Lactobacillus in mice with spleen-deficiency constipation

【目的】探讨超微铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的影响,为疗效提供依据。【方法】制备小鼠脾虚便秘模型,灌胃铁皮石斛传统汤剂、超微50%量汤剂治疗,采集肠道内容物提取肠道微生物宏基因组DNA,用乳酸杆菌特异引物PCR后进行ARDRA分析。【结果】结果显示,正常组、铁皮石斛传统汤剂组和超微50%量汤剂组的OTUs、Shannon指数和Brillouin指数相同,且均大于模型组;铁皮石斛超微50%量汤剂组与正常组乳酸杆菌群落结构的相似性系数最大,为0.333 3,其次是模型组为0.181 8,传统汤剂组最小,为0.166 7;聚类分析和主成分分析结果显示,小鼠肠道乳酸杆菌多样性受脾虚便秘造模影响,发生改变,铁皮石斛两种汤剂通过不同的途径对其进行调控作用。【结论】铁皮石斛对脾虚便秘小鼠肠道乳酸杆菌多样性的调整作用明显,且超微50%量汤剂组小鼠肠道乳酸杆菌多样性更接近正常组,疗效更优。

[Objective] To provide the basis for its treatment, the effect of ultra-micro powder Dendrobium officinale on the molecular diversity of intestinal Lactobacillus in mice with spleen-deficiency constipation was studied. [Methods] The mice with spleen-deficiency constipation in treatment groups were respectively given the traditional decoction of Dendrobium officinale and50% dose of ultra-micro powder Dendrobium officinale. The metagenome DNA of intestinalmicroflora was extracted from intestinal contents. Then ARDRA analysis was made after PCR by specific primer of Lactobacillus. [Results] The results showed that OTUs, Shannon index and Brillouin index in the normal group, the traditional decoction group and the 50% dose of ultra-micro powder group were the same, and all were larger than which in the model group. Compared with Lactobacillus community structure in normal group, the similarity coefficient was 0.333 3 in the 50%dose of ultra-micro powder group, 0.181 8 in the model group, 0.166 7 in the traditional decoction group. The cluster analysis and principal components analysis suggested that molecular diversity of intestinal Lactobacillus in mice changed by the effect of spleen-deficiency constipation model, and the two kinds of decoction of Dendrobium officinale regulated it in different ways. The Dendrobium officinale had obvious effect on the molecular diversity of intestinal Lactobacillus in mice with spleen-deficiency constipation. [Conclusion] The efficacy of 50% dose of ultra-micro powder Dendrobium officinale was the best in all treatment groups, and the molecular diversity of intestinal Lactobacillus in 50% dose of ultra-micro powder Dendrobium officinale group was closer to the normal group.