载银氨基硅胶柱分离EPA和DHA的研究

Separation of EPA and DHA by Ag^+ modified amino silica gel column

利用负载银离子的氨基硅胶为固定相,制备4.6 mm×250 mm载银氨基硅胶柱,系统评价EPA和DHA在正相体系中的色谱保留行为,考察了载银氨基硅胶的粒径和流动相对EPA和DHA分离的影响。结果表明:5μm粒径的载银氨基硅胶比10μm粒径的分离度好;随着流动相中正己烷比例的增大,EPA和DHA分离度增大;当选用5μm载银氨基硅胶、流动相为正己烷-异丁醇(体积比2 000∶1)、流速1 mL/min、紫外检测波长210 nm、进样量5μL时,EPA和DHA的分离度最佳,分离度为2.03。该方法简单、快速、重现性好,可用于EPA和DHA的分离。

The analysis column （4. 6 mm × 250 mm） for the separation of EPA and DHA was prepared with Ag＋ modified amino silica gel as stationary phase. A systematic evaluation on retention behavior of EPA and DHA in normal phase chromatography was performed. The influences of Ag＋ modified amino silica gel with different pore sizes and mobile phase on the separation of EPA and DHA were researched. The results showed that the separation degree of 5 μm Ag＋ modified amino silica gel was better than that of 10 μm Ag＋ modified amino silica gel;the separation degree of EPA and DHA increased with the pro-portion of n-hexane in mobile phase increasing;when the pore size of Ag＋ modified amino silica gel was 5 μm, the volume ratio of n-hexane to isobutanol was 2 000∶1, flow rate was 1 mL/min, ultraviolet de-tection wavelength was 210 nm and the injection volume was 5 μL, the separation degree of EPA and DHA was the best and reached 2. 03. The method had the items of simpleness, rapidness and high repro-ducibility, which was suitable for the separation of EPA and DHA.