柱前衍生HPLC法测定干髓糊剂中多聚甲醛的含量

Content Determination of Paraformaldehyde in Mummification Pastes by HPLC with Pre-column Derivatization

目的：建立测定干髓糊剂中多聚甲醛含量的方法。方法：采用2，4-二硝基苯肼柱前衍生高效液相色谱法。色谱柱为Diamonsil C18（2），流动相为甲醇．水（60：40，V/V，流速为1．0ml／min，检测波长为355nin，柱温为25℃。结果：多聚甲醛（以甲醛计）检测质量浓度线性范围为5．05-252．50μg／ml（r=0．9997）；精密度、重复性、稳定性试验的RSD均小于2％；平均加样回收率为99．12％，RSD=1．86％（n=9）。结论：建立的方法专属性强，结果准确可靠，可用于干髓糊剂中多聚甲醛的含量测定。

OBJECTIVE： To establish a method for the content determination of paraformaldehyde in mummification pastes. METHODS： HPLC with pre-column 2, 4-dinitrophenylhydrazine derivatization method was adopted. The determination was performed on Diamonsil C18 （2） column with mobile phase consisted of methanol-water （60 ： 40, V/F） at the flow rate of 1.0 ml/min. The UV detection wavelength was set at 355 nm and the column temperature was 25℃. RESULTS： The linear range of paraformaldehyde （calculated by formaldehyde） was 5.05-252.50 μg/ml （r=0.999 7）. RSDs of precision, reproducibility and stability tests were all lower than 2%. The average recovery was 99.12% （RSD=1.86%, n=9）. CONCLUSIONS： The method is specific, accurate and reliable, and it can be used for the content determination of paraformaldehyde in mummification pastes.