TiO2纳米颗粒注射昆明鼠的抗电离辐射作用研究

Anti-radiation effect of TiO2 nanoparticles given to Kunming mouse

目的 评价TiO2纳米颗粒对小鼠的辐射防护作用.方法 对6～8周龄昆明鼠,给予6.5 Gyγ-射线一次性全身照射.用不同剂量TiO2纳米颗粒10、20、40 mg/kg,在小鼠照射前2d、1d和照射后1d连续给药3次.在照射后第8天取外周血、胸腺、肝、脾、肺和两侧股骨.血液细胞分析仪分析白细胞数（WBC）、血小板数（PLT）和血红蛋白数（HGB）;计数股骨有核细胞（BMNC）和骨髓细胞DNA含量以及肝肺的超氧化物歧化酶（SOD）和丙二醛（MDA）含量;脏器称重计算相关的脏器系数,评价TiO2纳米颗粒的抗辐射损伤效应.结果 与空白对照组相比,除MDA外,各实验组的WBC、PLT、HGB、BMNC、SOD和骨髓细胞DNA指标均有所增加,且高剂量实验组与对照组的差异具有统计学意义（P＜0.05）.结论 高剂量TiO2纳米颗粒对6.5 Gy辐射损伤的昆明鼠有辐射防护作用.

Objective To evaluate the anti-radiation effect of TiO2 nanoparticles at different concentration levels on mice.Methods Kunming mice aged 6-8 weeks were selected to receive whole body radiation of 6.5 Gy γ-ray.The mice of experimental groups were treated with 10,20 and 40 mg/kg TiO2 nanoparticles respectively.Before radiation,the above mice received two consecutive days of treatment of intraperitoneal injection.Continuously,mice were administered by TiO2 nanoparticles for another post-radiation day.The white blood cell count （WBC）,platelet count （PLT）,hemoglobin count （HGB）,bone marrow nuclear cell （BMNC）,bone marrow DNA and superoxide dismutase （SOD）,malonaldehyde （MDA） of liver and lung were counted and the organs indices were calculated by weighing liver,spleen and thymus to assess the radiation-protection effect of TiO2 nanoparticles.Results Compared with those in the control group,the parameters of treatment groups （excluding MDA） increased and statistical difference between the high-dose groups and control mice were detected （P＜0.05）.Conclusions TiO2 nanoparticles injection showed a short-term radiation-protection effect on Kunming mice and the effect of high-dose is better than low-and middle-dose.