不同产地云白芍中3种活性成分含量的高效液相色谱法测定

Content determination of 3 active ingredients in Paeonia delavayi Franch. from different producing area by HPLC

目的 同时测定不同产地云白芍药材中儿茶素、芍药苷和苯甲酰芍药苷的含量。方法 采用ZORBOX SB-C18柱（4.6 mm×250 mm,5μm）;流动相为乙腈（A）-0.1%甲酸溶液（B）,梯度洗脱：洗脱条件为：0~2 min,5%A;2~30 min,5%A→16%A;30-50 min,16%A→20%A;50-75 min,20%A→60%A;流速1.0ml·min-1;检测波长245 nm;柱温30℃。结果 儿茶素、芍药苷和苯甲酰芍药苷分别在0.200-2.000μg（r=0.999 3）、0.202-2.020μg（r=0.999 5）、0.200-2.000μg（r=0.999 8）范围内呈良好的线性关系;平均回收率（n=6）分别为98.12%（RSD=1.66%）、99.44%（RSD=0.78%）、97.25%（RSD=1.55%）。结论 该方法准确可靠、重复性好,是用于云白芍的质量控制的有效方法之一;不同产地云白芍不同部位中儿茶素、芍药苷和苯甲酰芍药苷的含量差异较大。

Objective To determined contents of catechin acid,peoniflorin and benzoylpaeoniflorin in medicinal materials of Paeonia delavayi Franch. from different producing areas. Methods The samples were separated by a ZORBOX SB-C18（4.6 mm ×250 mm,5 μm） using acetonitrile（A）-0. 1% methane acid（B） with water gradient system as mobile phase. Elution condition was as the following： 0 ~ 2 min,5 % A; 2 ~ 30 min,5 % A→16 % A; 30 ~ 50 min,16 % A→20 % A; 50 ~ 75 min,20 % A→60%A. The flow rate was 1. 0 ml·min-1and the detection wavelength for fingerprinting was 245 nm with 30℃ for column temperature. Results There were good linear relationships for catechin acid,peoniflorin and benzoylpaeoniflorin in range of 0. 200 - 2.000 μg（r = 0. 9993）,0. 202 ~ 2. 020 μg（r = 0. 9995） and 0. 200 ~ 2. 000 μg（r = 0. 9998）. And average recovery rates for them（n = 6） were 98. 12%（RSD = 1. 66%）,99. 44%（RSD = 0. 78%） and 97. 25%（RSD = 1. 55%）. Conclusion The method could be used for quality control of medicinal materials of P. delavayi with well accuracy and repeatability. There was significant difference for contents of catechin acid,peoniflorin and benzoylpaeoniflorin in the medicinal materials from different areas.