葡萄糖调节蛋白78和基质金属蛋白酶在口腔鳞状细胞癌中的表达

Expression of Glucose Regulated Protein 78 and Matrix Metalloproteinases in Oral Squamous Cell Carcinoma

目的探讨葡萄糖调节蛋白78(GRP78)和基质金属蛋白酶(MMPs)在口腔鳞状细胞癌组织中的表达及意义,阐明GRP78在口腔鳞状细胞癌发生、发展中的作用。方法采用免疫组织化学方法检测26例口腔鳞状细胞癌组织和10例正常口腔组织中GRP78蛋白的表达;采用逆转录PCR检测正常口腔组织和口腔鳞状细胞癌组织中GRP78 m RNA的表达;采用免疫组织化学方法检测26例口腔鳞状细胞癌组织中MMP-2和MMP-9蛋白的表达。结果 GRP78蛋白在口腔鳞状细胞癌组织中的阳性表达率为100%(26/26),在正常口腔组织中的阳性表达率为20%(2/10),两者比较差异有统计学意义(P<0.01);与高分化和中分化口腔鳞状细胞癌组织比较,低分化口腔鳞状细胞癌组织中的GRP78蛋白阳性表达强度明显增高(P<0.05)。与正常口腔组织比较,GRP78 m RNA表达水平在口腔鳞状细胞癌组织中的表达增高(P<0.05)。MMP-2和MMP-9蛋白在口腔鳞状细胞癌组织中的阳性表达率均为100%(26/26)。结论 GRP78和MMPs在口腔鳞状细胞癌组织中表达明显升高,提示口腔鳞状细胞癌中GRP78蛋白的表达可能与肿瘤的侵袭和转移相关。

Objective To explore the expression and significance of glucose regulated protein 78 （GRP78） and matrix metalloproteinases （MMPs） in human oral squamous cell carcinoma, and clarify the effect of GRP78 in the process of oral squamous cell carcinoma. Methods The ex- pression of GRP78, MMP-2 and MMP-9 were evaluated by immunohistochemistry in 26 oral squamous cell carcinoma tissues. RT-PCR was per- formed to detect the mRNA levels of GRP78 in oral squamous cell carcinoma tissues. Results The results of immunohistochemistiy indicated that the positive rates of GRP78 were 100% and 20% in oral squamous cell carcinoma tissues and normal oral tissues, and the difference was significant （P 〈 0.01 ）. Compared with well differentiated and moderately differentiated oral squamous cell carcinoma tissues, the positive rates of GRP78 pro- tein in poorly differentiated oral squamous cell carcinoma tissues were obvious higher （P 〈 0.05 ）. Compared with normal oral tissues, the expression of GRP78 mRNA in oral squamous cell carcinoma tissues was significantly increased （P 〈 0.05 ）. Compared with well differentiated and moderately differentiated oral squamous cell carcinoma tissues, the expression of GRP78 mRNA in poorly differentiated was significantly increased （ P 〈 0.05 ）. The positive expression of MMP-2 and MMP-9 were 100% in oral squamous cell carcinoma tissues. Conclusion The expression of GRP78 and MMPs were obviously increased in oral squamous cell carcinoma tissue. The expression of GRP78 may plays an important role in the development and progression of oral squamous cell carcinoma.