利用RFLP分析DO对附积床系统中AOB群落结构的影响

Analysis of ammonia-oxidizing bacteria in catching bed reactor at different DO concentrations by RFLP

为了解析DO浓度对附积床反应器脱氮系统中COD、NH^4＋-N、TN去除效率的影响,以及对氨氧化菌群（AOB）结构及多样性的影响,分析了DO分别为1.0～2.0,2.0～3.0,3.0～4.0mg/L时COD、NH^4＋-N、TN去除效率,并采用针对AOB功能基因氨单加氧酶（amoA）的限制性内切酶片段长度多态性技术（RFLP）分析了三组DO浓度下反应器中AOB的群落结构及多样性.结果表明,不同DO条件下,系统均取得较高的COD和NH^4＋-N的去除效果, NH^4＋-N的去除效率随着DO的增加而提高.不同DO浓度下反应器生物膜上AOB菌群多样性丰富,且与DO对AOB菌群的多样性影响较小相比,DO对AOB的菌群结构及种类的影响较大.

To investigate the influence of DO concentration on the characteristics of catching bed reactor, the removal efficiency of COD, NH^4＋-N and TN were analyzed under the DO concentrations at 1.0～2.0mg/L, 2.0～3.0mg/L and 3.0～4.0mg/L, respectively. Meanwhile, ammonia-oxidizing bacteria （AOB） community and diversity in each reactor were examined using restricted fragment length polymorphism （RFLP）, and sequencing of amoA genes. The results showed that higher COD and NH^4＋-N removal efficiency were received under different DO concentrations, NH^4＋-N removal efficiency increased with the DO concentration increase. The diversity of AOB was abundant in different DO concentrations, but the communities and species of AOB were effect greatly by DO concentration.