壬基酚对大鼠5-羟色胺分解代谢通路的影响

5-Hydroxytryptamine catabolic changes of rats by short-term exposure to nonylphenol

为探讨壬基酚对5-羟色胺(5-HT)代谢网络分解代谢通路的毒性作用机制,将24只SD雄性大鼠分为阴性对照组和壬基酚低、中、高剂量组[30,90,270mg/(kg·bw)],隔日玉米油灌胃染毒28d后检测大鼠血浆及肝匀浆中单胺氧化酶(MAO)活性和血浆中5-HT含量,并称量肝、肾和睾丸重量.结果显示,壬基酚暴露高剂量组大鼠体质量增量极显著低于对照组(P<0.01);染毒至第3周起,各组大鼠平均食物利用率有统计学意义(P<0.05);与对照组相比,高剂量组肝脏、肾脏脏器系数均有统计学意义(P<0.01),中、高剂量组睾丸脏器系数均有统计学意义(P<0.05);中、高剂量组血浆MAO酶活性极显著低于对照组(P<0.01),中、高剂量组肝匀浆MAO酶活性显著低于对照组(P<0.05);中、高剂量组血浆5-HT含量极显著高于对照组(P<0.01).壬基酚暴露剂量与大鼠体内MAO酶活性及5-HT水平均呈剂量-效应关系,壬基酚可通过分解代谢通路影响5-HT的代谢网络.

Effects of nonylphenol （NP） on monoamine oxidase （MAO） activity and 5-hydroxytryptamine （5-HT） level of rats were investigated. The toxic mechanisms of NP on catabolism of 5-HT were also analyzed. 24 male SD rats were randomly divided into 4groups, namely NP low-dose group 30mg/（kg·bw）, NP medium-dose group 90mg/（kg·bw）, NP high-dose group 270mg/（kg·bw） and control group （equal volume of the solvent）. NP in corn oil was administrated orally to rats once every other day for 28days, and rats in control group was only given corn oil. The increment of rat body weight in NP high-dose group was lower than that of control group （P〈0.01）. From the third week of exposure, significant differences were found for the food utilization rates, the liver, kidney and testis coefficients between treatment groups and control group （P〈0.05, P〈0.01, P〈0.01, P〈0.05）. Compared with control group, significantly lower MAO activities of plasma and liver homogenate and higher 5-HT levels were observed in treatment groups （P〈0.01, P〈0.05, P〈0.01）. NP exposure has a dose-dependent effect on MAO activity and 5-HT level of rats, suggesting that NP may affect the metabolic network of 5-HT by inhibiting its catabolism.