直接进样离子色谱法测定金银花中的4种阳离子含量

Determination of Four Kinds Cation Content in Jinyinhua by Direct Injection Ion Chromatography

目的：建立直接进样离子色谱法测定中药金银花超声提取液中的Na＋、K＋、Ca2＋、Mg2＋4种阳离子的含量方法。方法：选用ICS-900离子色谱IonPac CS12A阳离子色谱柱,20mmol/L MSA等度淋洗脱,电导检测金银花中的4种阳离子。结果：Na＋、K＋、Ca2＋、Mg2＋的线性范围良好,最低检出限为0.020、0.025、0.015和0.050 mg/L,样品加样回收率介于97%-102%之间。结论：该法操作简便、快速、准确,为金银花中的Na＋、K＋、Ca2＋、Mg2＋含量测定提供了一种有效的方法。

To establish determination methods of Na＋,K＋,Ca2＋ and Mg2＋ four kinds of cation content in Jinyinhua ultrasonic extract by Direct Injection Ion Chromatography. Methods：We choosed ICS-900 ion chromatography IonPac CS12A cation column and 20 mmol/L MSA isocratic eluting,and detected four kinds of cation content in Jinyinhua by conductivity. Results：The linear range of Na＋,K＋,Ca2＋,Mg2＋ was good. The detection limit was 0.020,0.025,0.015 and 0.050. Sample recoveries was from 97% to 102%. Conclusion： This method is simple,rapid,accurate,and provided an effective determination method for Na＋,K＋,Ca2＋ and Mg2＋ determination in Jinyinhua