小儿血液系统疾病人细小病毒B_(19)感染的研究

The investigation of HPV B_(19) infection on children with blood system diseases

目的:探讨我国西北地区血液系统疾病患儿人细小病毒B19(HPV B19)感染情况及两者的相关性。方法:对特发性血小板减少性紫癜、再生障碍性贫血和白血病患儿的外周血分别采用PCR方法检测HPV B19-DNA和ELISA方法检测HPV B19-IgG。结果:特发性血小板减少性紫癜患儿HPV B19-IgG阳性率为43.08%(28/65),白血病患儿HPV B19-IgG阳性率为41.07%(46/112),病例组较对照组比较有极显著性差异(P<0.01);再生障碍性贫血患儿HPV B19-IgG阳性率为21.43%(6/28),病例组较对照组比较有显著性差异(P<0.05)。65例特发性血小板减少性紫癜患儿HPV B19-DNA阳性率为40.00%(26/65);28例再生障碍性贫血患儿HPV B19-DNA阳性率为21.43%(6/28);112例白血病患儿HPV B19-DNA阳性率为27.68%(31/112);对照组HPV B19-DNA检测均为阴性。结论:HPV B19感染与我国西北地区部分血液系统疾病患儿发病相关。

Objective：To investigate the relationship between human parvovirus B 19 （HPV B19 ） infection and children who had blood system disease .Methods ：PCR and ELISA methods were used to detecte HPV B19-DNA and HPV B19-IgG in child who had idiopathic thrombocytopenic purpura ,aplastic anemia or leukemia .Results ：The positive rate of HPV B19-IgG was 43 .08% in patient with idiopathic thrombocytopenic purpura ,the positive rate of HPV B19-IgG was 41 .07% in patient with leukemia ,and the positive rate of HPV B19-IgG was 21 .43% in patient with aplastic anemia .There were statistical differences between the experiment groups and the control group .The positive rate of HPV B19-DNA was 40 .00% in patient with idiopathic thrombocytopenic purpura ,the positive rate of HPV B19-DNA was 27 .68% in patient with leukemia ,and the positive rate of HPV B19-DNA was 21 .43% in patient with aplastic anemia .Conclusion ：HPV B19 infection have relationship with some children who have blood system dis-eases in northwest region of China .