胰高血糖素样肽-1对大鼠糖溶液摄入的影响

Glucagon-like peptide-1 modulate sucrose solution intake in rats

目的:激活外周胰高血糖素样肽-1(GLP-1)受体,观察大鼠对可口蔗糖溶液摄入量及其喜好率的变化,探讨其参与摄食调控的可能机制。方法:给予大鼠腹腔注射Exendin-4(GLP-1受体激动剂)0.5μg/100g或同量生理盐水,利用双瓶选择实验,测量大鼠对8%蔗糖溶液/蒸馏水的摄入量,计算对蔗糖溶液的喜好率。结果:Exendin-4组与生理盐水组相比,大鼠摄入8%蔗糖溶液的量、对蔗糖溶液的喜好率以及3h总液体摄入量均显著减少(P<0.05),但对蒸馏水的摄入无显著影响(P>0.05)。结论:激动GLP-1外周受体后,可降低大鼠对蔗糖溶液的喜好程度,进而抑制对可口溶液的摄入。

Objective： To explore the effects of the periphery glucagon-like peptide-l（GLP-1） receptors in modulating sucrose solution intake in rats. Methods： The rats received intraperitoneal injection of GLP-1 receptors agonist exendin-4 or saline, and then underwent two bottle choice test between 8% sucrose solution and distilled water. We measured the sucrose solution/distilled water intake and preference ratio of sucrose solution. Results：Compared with saline injection, intraperitoneal injection of exendin-4 significantly decreased 8% sucrose solution intake, the preference ratio of sucrose solution and the total fluid intake in rats during 3h, but had no effect on the distilled water intake. Conclusion： These results suggest that activation of the periphery GLP-1 receptors may be involved in inhibition of the tasty sucrose solution intake by reducing the preference for it.