革兰氏阴性益生菌大肠杆菌Nissle 1917益生机理及其在仔猪方面的应用

Probiotic Mechanism of Gram Negative Probiotics E. coli Nissle 1917 and Its Application in Piglets Raising

革兰氏阴性菌大肠杆菌(E.coli)Nissle 1917(EcN)是非乳酸益生菌中研究最多的益生菌。EcN血清型为O6∶K5∶H1,具有独特的基因组、半粗糙O6-脂多糖(LPS)表型、K5型荚膜、3种不同的菌毛(F1A、F1C和卷曲菌毛)。EcN具有小菌素和铁摄取系统等特殊适应性因子,在与其他微生物竞争中起关键作用。EcN能长期稳定定植于肠道,与肠上皮细胞相互作用,发挥免疫调节生物学功能。本文就EcN的特征、定植机制、免疫调节机理和在仔猪方面应用的研究现状进行综述,以期为益生菌EcN和其他益生革兰氏阴性杆菌在动物方面研究和应用提供参考。

A gram-negative strain E. coli Nissle 1917 （EcN） is the most researched probiotic except lactic acid bacteria. EcN, serotyping of O6：K5：H1, has special genome, presents semi-rough O6-1ipopolysaccharide （LPS） phenotype, K5 type and 5 different fimbriae （ FIA, F1C and a kind of curli fimbriae capsule）. The iron uptake system and the microcin of EcN, acting as their ＂fitness factor＂, are essential to antagonizing other bacteria. EcN has long-term steady colonization in the gut, interact with intestinal epithelial cells and develop immunomodulation biological function. This paper reviewed the futures, colonization mechanism, immuno- modulation mechanism of EcN and its application in piglets raising, which will provide reference for further study on EcN and other probiotic gram-negative bacteria in animal intestine.