摘要
目的:建立广升麻的HPLC指纹图谱,为广升麻的质量控制提供依据。方法:采用高效液相色谱法,以β-蜕皮甾酮为内参照物,采用色谱柱Agilent C18(3.0 mm×100 mm,1.8μm),乙腈-0.1%甲酸溶液梯度洗脱,流量为0.4 m L·min-1,检测波长为310 nm,柱温30℃。结果:获得了较为理想的广升麻药材HPLC指纹图谱,确定了13个共有峰。结论:该方法简便、准确、可靠,为评价广升麻药材的质量提供依据。
Objective：To establish HPLC fingerprint of Radix Serratulae Chinensis.Methods： β-ecdysone was used as the internal standard substance.The reversed phase HPLC system was consisted of a C18 column （3.0 mm ×100 mm,1.8 μm） and a mixture of acetonitrile-0.1%formic acid solution as the mobile phase with gradient elution mode at the flow rate of 0.4 mL· min-1.The absor-bance was monitored at 310 nm and the column temperature was 30℃.Results：A satisfactory method for HPLC fingerprint determina-tion of Radix Serratulae Chinensis was established , 13 characteristic peaks in the HPLC fingerprint were indicated.Conclusion：The method is simple , accurate with good repeatability and can be used as a quality control method for Radix Serratulae Chinensis .
出处
《中国药品标准》
CAS
2014年第4期262-265,共4页
Drug Standards of China
