HMGB1及α-SMA在支气管肺发育不良新生儿中的表达及临床意义

Expression and clinical significance of HMGB1 and α-SMA in neonatus with bronchopulmonary dysplasia

目的:观察高迁移率族蛋白B1(HMGB1)、α-平滑肌肌动蛋白(α-SMA)在支气管肺发育不良(BPD)新生儿血清、肺泡灌洗液中的表达,并探讨其临床意义。方法:采用Elisa法检测76例BPD新生儿(急性期18例,再生期17例,过渡期21例,慢性期20例)和24例非BPD新生儿血清、肺泡灌洗液HMGB1及α-SMA的表达水平,分析两者之间的相关性。结果:观察组各时期血清、肺泡灌洗液HMGB1、α-SMA表达水平均高于对照组(P<0.05),随病理进展血清、肺泡灌洗液HMGB1、α-SMA表达水平呈明显升高趋势(P<0.05);两组血清、肺泡灌洗液HMGB1与α-SMA表达均呈正相关性关系(P<0.05)。结论:血清及肺泡灌洗液HMGB1、α-SMA表达可作为诊断新生儿BPD的有效实验室指标,具有重要的临床意义。

Objective： To observe the expression of high mobility group protein B1（HMGB1）,α-smooth muscle actin（α-SMA） in serum and bronchoalveolar lavage fluid of neonatus with bronchopulmonary dysplasia（BPD）,and to explore its clinical significance. Methods： The expression of HMGB1,α-SMA in serum and bronchoalveolar lavage fluid of 76 cases of neonatus with BPD（ acute period,18 cases,regeneration period： 17 cases,transition period： 21 cases,chronic period： 20 cases） and 24 cases of non-BPD were detected by Elisa,and analyzed the correlation between HMGB1 and α- SMA. Results： The expression of HMGB1, α- SMA in serum and bronchoalveolar lavage fluid of observation group in every periods were higher than that of the control group（ P 〈0. 05）. With the pathological progress,the expression of HMGB1,α-SMA in serum and bronchoalveolar lavage fluid was significantly increased（P 〈 0. 05）. There was a positive correlation relationship between the expression of HMGB1 and α-SMA in serum and bronchoalveolar lavage fluid（ P 〈 0. 05）. Conclusion： The expression of HMGB1,α-SMA in serum and bronchoalveolar lavage fluid could be used as an effective index to diagnose neonatus with BPD. It has an important clinical significance.