SSP-PCR方法检测HLA-B27基因在AS早期诊断中的临床应用

Clinic Application about Detect HLA-B27 Gene by SSP-PCR in AS Early Diagnosis

目的 验证分子学方法序列特异引物聚合酶链反应（SSP-PCR）在强制性脊柱炎（AS）早期诊断中的临床应用.方法 收集陕西省人民医院2013年1月～12月疑似强直性脊柱炎患者135例,分别采用SSP PCR方法检测全血人类白细胞抗原B27位点（HLA-B27）和进行影像学骨盆、骶髂关节的X线、CT或者MRI检查,比较SSP PCR方法和影像学方法诊断的一致性及差异性.结果 疑似患者用SSP PCR方法检测HLA-B27的阳性检出率为37.0％,而影像学方法的诊断异常率为23.7％,SSP-PCR方法的阳性率较影像学方法高出13.3％.经配对X2检验,X2=9.529,P=0.003,＜0.01,两种方法阳性检出率差异具有统计学意义.结论 分子学方法检测HLA-B27基因能够用于早期预测AS的发生.

Objective To verify clinical application of sequence specific primer PCR （SSP PCR） method to detect HLA-B27 in early diagnosis ankylosing spondylitis （AS）.Methods Collected 135 cases of suspected AS patients in Shaanxi Provincial People＇s Hospital from January 2013 to December 2013.Detected human leukocyte antigen B27 （HLA-B27） by SS P-PCR method in full blood,and checked pelvis,sacroiliac joint by photography method such as X-ray,CT and MRI respectively.Compared diagnostic consistency and difference between SSP-PCR method and photography method.Results SSP-PCR method for detecting HLA-B27 positive rate was 37.0％ in suspected patients,while the abnormal rate was 23.7％ with photography method and positive rate in SSP-PCR method was 13.3％ higher than photography method.After pairing x2 test,two methods positive rate difference was statistically significant （x2 =9.529,P=0.003,P＜0.01）.Conclusion SSP-PCR method to detect HLA-B27 gene can be used for early predication of AS occurrence.