136例急性白血病免疫表型特点及临床意义

Immunophenotype Characteristics and Clinical Significance of 136 Patients with Acute Leukemia

目的 探讨急性白血病免疫表型的特点及临床意义.方法 采用碱性磷酸酶抗碱性磷酸酶（APAAP）方法对136例急性白血病进行免疫表型分析.结果 136例急性白血病中,97例急性髓细胞白血病（AML）主要表达抗髓过氧化物酶MPO（95.87％）,CD68 （89.69％）,CD13 （86.59％）和CD33 （82.47％）;其次为HLA-DR（61.85％）和CD34 （57.73％）.16.49％的AML患者伴淋系抗原表达,最常见为CD7 （9.27％）,CD19 （6.18％）及CD2 （3.09％）.B细胞系急性淋巴细胞白血病（ALL） 28例,主要表达CD19（100％）,HLA DR（96.42％）,CD22（75％）,CD34（71.42％）及CD10 （67.85％）.T细胞系ALL 11例,主要表达CD7（100％）,CD3（81.81％）及CD5 （72.72％）.17.94％ALL患者表达髓系相关抗原,主要是CD13（15.38％）及CD33（10.71％）.结论 免疫表型检测能提高急性白血病诊断率,并对亚型鉴别有重要意义.

Objective To investigate the immuneophenotype characteristics and clinical significance in acute leukemia.Methods Immunophenotyping was performed by alkaline phosphatase anti alkaline phosphatase（APAAP） method.Results In 97 patients of 136 with acute Myeloid leukemia（AML）,the antigens were mainly MPO（95.87％）,CD68 （89.69％）,CD13 （86.59 ％）,CD33（82.47 ％）,HLA DR（61.85 ％）,CD34（57.73 ％）.16.49％ patients were accompanied with lymphocyte antigens CD7 （9.27 ％）,CD19 （6.18 ％） and CD2 （3.09 ％）.In 28 patients with B Lineage acute lymphoblastic leukemia,the antigens were mainlyCD19（100％）,HLADR（96.42％）,CD22（75.00％）,CD34（71.42％） and CD10（67.85％）.In 11 patients with T lineage acute lymphoblastic leukemia,the antigens were mainly CD7 （100％）,CD3 （81.81％） and CD5 （72.72％）.Myeloid associated antigen expressed in 17.94％ of the ALL especially CD13 （15.38％） and CD33（10.71％）.Conclusion Immunophenotyping is extremely important in diagnosis and subtype differentiation of acute leukemia.