血清学试验联合葡萄糖检测在污染菌判断中的应用

Judgment of the Contaminated Bacteria by Using Serological Test Combined Glucose Detection

目的 利用血清葡萄糖检测以及血清凝集反应的原理判断培养基中的菌株是否为污染菌.方法 选取2011年5月～2012年11月272例细菌培养阳性且所分离菌株能分解葡萄糖的病例,于病程的初、中、末三期采集患者血清分别进行如下试验：将所选取经常规方法分离培养出并已确定为致病菌的菌株和非来自患者的其它菌株（污染菌）分别配制成2.5 MCF菌悬液.在一定条件下,分别将上述两种菌悬液与患者定量血清进行凝集反应,并将最终所余反应物进行葡萄糖检测以确定各自参考区间.结果 病原菌（2B/A）三期的区间范围分别为：0.772 5±0.102 0,0.452 5±0.088 9和0.297 5±0.044 2;污染菌（2C/A）三期的区间范围分别为：0.012 9±0.006 5,0.012 5±0.006 7和0.012 7±0.006 3.应用SPSS11.5统计软件进行t检验,分别将2B/A与2C/A的三组数据进行比较,2B/A值在抗生素应用各期差异有统计学显著性意义（F=181.351,P＜0.01）;而2C/A值在抗生素应用各期差异无统计学意义（F=0.291,P＞0.05）.结论 上述区间范围表明,病原菌与污染菌在该试验中相关数据具有明显差异,由此可制定出污染菌判断的参考区间.

Objective To determine the strains in the culture medium are contaminating bacteria or not by using the principle of serum glucose test and serum agglutination.Methods From 272 positive bacterial cultures and the strains isolated can breakdown of glucose,the cases were collected from May 2011 to November 2012.Collected the serum of patients in the prophase,metaphase and anaphase of the course,and the following experiments had been done respectively：the strains of pathogenic bacteria identified by conventional methods and the other strains not from patients were both made into 2.5 MCF bacteria suspension.The agglutination reactions were carried out by using the two bacterial suspensions above with the host serum quantified respectively under certain conditions,and detected the glucose in the leftover reactant to make the reference ranges for each.Results The ranges of the three stages of pathogens （2B/A） were 0.772 5±0.102 0,0.452 5±0.088 9 and0.297 5±0.044 2,and those of the contaminating bacteria （2C/A） were 0.012 9±0.006 5,0.012 5±0.006 7 and 0.012 7±0.006 3.Compared the the three sets of data of 2B/A and 2C/A by the t test of SPSS11.5 software,differences were significant in each period of 2B/A in application of antibiotics （F=181.351,P＜0.01）,and there was no significant differences in that of 2C/A（（F=0.291,P＞0.05）.Conclusion There was significant interval difference between pathogens and contaminating bacteria,and by that the reference range of pollutions of bacteria can be made.