慢性乙肝病毒感染产妇新生儿脐血树突状细胞亚群变化与乙肝病毒核酸载量的关系

Relationship between HBV DNA and cord blood dendritic cells subset isolated from fetuses whose mothers had chronic hepatitis B

目的：观察慢性乙型肝炎病毒感染产妇新生儿脐血中树突状细胞亚群（DC ）的变化与乙肝病毒核酸（HBV DNA）载量的关系。方法采集28例慢性乙型肝炎病毒感染产妇所生新生儿脐血，用三色分析法进行免疫荧光标记，24 h内上流式细胞仪进行树突状细胞的检测。同时对慢性乙型肝炎感染的新生儿脐血分离血清，进行HBV DNA载量的检测。结果慢性HBV感染产妇和健康产妇新生儿脐血DC1、DC2和DC1/DC2比较，差异均无统计学意义；慢性HBV感染产妇新生儿脐血HBV DNA阴性（HBVDNA＜103拷贝/ml）、阳性（HBVDNA≥103拷贝/ml）分别为57.1％和42.9％；HBV DNA≥106拷贝/ml、HBV DNA＜106拷贝/ml分别为41.7％和58.3％；HBV DNA阳性者DC1、DC2低于HBV DNA阴性者，脐血HBV DNA阳性中HBV DNA＞106拷贝/ml者的DC1和DC2相对数均明显低于HBV DNA＜106拷贝/ml者，差异均有统计学意义。结论慢性乙型肝炎病毒感染产妇新生儿并未全部感染乙肝病毒；慢性乙型肝炎病毒感染产妇新生儿脐血中树突状细胞亚群相对数与脐血中HBV DNA含量有关，母血清HBV DNA含量≥106拷贝/ml时，胎盘感染乙肝病毒的危险性显著增加。

Objective Tocharacterizethevariationsofcordblooddendriticcells（DC）subsetisolatedfrom fetuses whose mothers had chronic hepatitis B （CHB）,to examine the relationships between these variations and HBV load,and to elucidate the transmission mechanism of CHB from mothers to their babies. Methods We detected 28 cord blood samples from fetuses whose mothers had chronic hepatitis B for the DC subsets.All the cord blood samples were detected immediately after being collected,and the percentages of DC1 and DC2 were determined by three-color cytometry. The samples from fetuses whose mothers had chronic hepatitis B were centrifuged,and assessed for HBVDNA load. Results In all of the 28 samples of fetuses whose mothers had chronic hepatitis B,12 was positive,and the others was negative.In the samples with HBV DNA positive,we found that the percentages of DC1 and DC2 were lower in cord blood whose HBV DNA was positive than it was negative. Conclusion Not all of the newborns were infected although their mothers had chronic hepatitis B.The relative quantity of the DC1 and DC2 in cord blood whose mothers had CHB was associated with HBV DNA level.