摘要
针对传统环氧氯丙烷生产工艺中二氯丙醇皂化过程产生大量高碱度、高含盐量有机废水问题,提出利用生物法转化二氯丙醇合成环氧氯丙烷的绿色工艺。利用表达重组卤醇脱卤酶的大肠杆菌全细胞为生物催化剂,考察了pH、温度、全细胞菌体添加量、底物浓度等对生物转化过程的影响。结果表明,优化的工艺条件为:最适pH为8.3,最适反应温度为45℃,全细胞菌体添加量为10 mg(DCW)/mL,底物浓度为20 mmol/L,在此条件下转化8 h后,1,3-二氯丙醇的最高转化率可达92.3%。全细胞生物催化剂重复反应3次后活力仍保留在80%以上,显示了其良好的应用前景。
In views of the emissions of a large number of high alkalinity and high salinity organic wastewater from the dichloropropanol saponifying process in traditional epichlorohydrin production, a green technology about using biotransformation of dichloropropauol to synthesize epichlorohydrin is presented. With a recombinant E. coli BL21 (DE3) expressing halohydrin dehalogenase as whole-cell biocatalyst, the influences of pH, the temperature of reaction, the amount of cells and substrates concentration are studied in detail. The optimum conditions for biotransformation are as follows :8.3 of pH, 45℃ of temperature, 10 mg DCW/mL of cells concentration, 20 mmol of substrate concentration. Under the optimal conditions, the conversion rate can reach 92. 3% after 8 hours' reaction. The whole-cell biocatalyst has good operational stability, suggesting the more prospective for application.
出处
《现代化工》
CAS
CSCD
北大核心
2014年第9期43-46,共4页
Modern Chemical Industry
基金
国家重点基础研究发展计划(2011CB710806)
国家自然科学基金(21176224)
教育部博士点新教师基金(2012331712004)
关键词
卤醇脱卤酶
全细胞生物转化
二氯丙醇
皂化废水
环氧氯丙烷
halohydriu dehalogenase
whole-cell biotransformation
dichloropropanol
saponification wastewater
epichlorohydrin