京尼平苷调节胰腺β细胞增殖的细胞信号机制研究

Effect of geniposide on proliferation of pancreatic β cells and its mechanisms

目的考察京尼平苷(Gen)对胰腺β细胞增殖的影响及其细胞信号转导机制。方法将INS-1细胞(一种胰腺β细胞)用Gen处理一定时间之后,用MTT方法测定活细胞的数量,用BrdU标记的方法分析细胞增殖的情况,Western印迹分析Gen对于细胞增殖相关的Akt和FoxO1磷酸化的影响。结果Gen处理INS-1细胞可将活细胞数量提高45%(P<0.01),并且BrdU标记的阳性细胞数量明显增加(P<0.01)。另外,Gen还能显著上调INS-1细胞中Akt308丝氨酸位点和叉头转录因子FoxO1的磷酸化(P<0.01),而且这种作用可被PI3K的抑制剂LY294002阻断。结论Gen能够促进胰腺β细胞增殖,而且这种作用可能与其激活PI3K激酶途径、增强FoxO1的磷酸化有关。

Objective To explore the role of geniposide （Gen） in the proliferation of pancreatic βcells and the cell signaling transduction pathway .Methods After INS-1 pancreatic βcell line was treated with indicated concentrations of Gen for 24 hours, the number of viable cells was measured with MTT assay , and the cell proliferation was evaluated with BrdU labeled method .Additionally , Western blotting was used to probe the phosphorylation of Akt 308 and FoxO1 induced by Gen.Results The number of INS-1 cells and BrdU labeled positive cells was increased by Gen .Moreover, the phospho-rylation of Akt308 and FoxO1 was induced , and the phosphorylation of FoxO 1 induced by Gen could be prevented in the presence of LY294002, an inhibitor of PI3K kinase.Conclusion Gen induces the proliferation of INS-1 cells in a dose-dependent manner, and PI3K signal pathway plays an essential role in the phosphorylation of FoxO 1 induced by Gen.