辣椒素乳膏中辣椒素含量的HPLC法测定

Determination of Capsaicin in Capsaicin Ointment by HPLC

用HPLC法以Phenomenex C18色谱柱（150mm±4.6mm）为固定相,甲醇-水（70∶30）为流动相，流速1.0ml·min-1，进样量20μl，检测波长为280 nm，按外标法以峰面积计算测定辣椒素乳膏中辣椒素的含量。结果辣椒素在52.308μg·ml-1-98.804μg·ml-1范围内线性关系良好，相关系数 r＝0.9999。平均回收率99.43%，RSD为0.85%。本法简便、准确、可靠,适用于辣椒素乳膏中辣椒素的含量测定。

High performance liquid chromatography was performed on Phenomenex C18 column. The chromatographic conditions were as follows： methanol- water（70：30） as mobile phase, flow rate being 1.0 ml·min-1,the detecting wavelength at 280nm. Capsaicin had good linear relation within the range from 52.308μg·ml-1 to 98.804μg·ml-1 （r=0.9999）. The average recovery was 99.43%, the repetitive RSD was 0.85%. The method is simple, rapid and accurate, which can be used for content determination of capsaicin in capsaicin ointment.