炎性因子在脑缺血——再灌注不同时期的基因表达

The gene expression of pro-inflammatory cytokines at different durations of cerebral ischemia reperfusion

目的 探讨白介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）和细胞间黏附分子1（ICAM-1）在脑缺血一再灌注不同时期的基因表达。方法 将Wistar大鼠随机分为假手术组和脑缺血30 min再灌注6 h（IR6h）、12h（IR12h）、24 h（IR24h）、48 h（IR48h）组。动物模型采用线栓法制备大鼠中动脉栓塞法,用RT-PCR法检测脑缺血一再灌注不同时间点IL-1β、TNF-α及ICAM-1的表达。结果 IL-1β、TNF-α及ICAM-1在假手术组脑组织内低水平表达。IL-1βmRNA的表达在脑缺血—再灌注6 h后即明显增高,12 b后表达开始减弱,24~48 h呈低水平增高。TNF-α在脑缺血—再灌注6 h表达开始上升,24 h达到高峰,48 h后开始下降。ICAM-1脑缺血—再灌注6 h表达开始升高,24 h达到高峰,48 h后仍维持较高水平。结论 炎症因子参与了脑缺血—再灌注性损伤,且具有明显的时间规律。

Objective To explore the gene expression of pro-inflammatory cytokines after different durations of cerebral ischemia reperfusion. Methods Rats were randomly divided into five groups：sham group, 30min of cerebral ischemia after 6h, 12h, 24h and 48h groups. Transient focal ischemia/reperfusion model in male Wistar rats were induced by middle cerebral artery occlusion（MCAO） according to the intraluminal filament technique as described. The expression of pro-inflammatory cytokines including interleukin-1β（IL-1β）, tumor necrosis factor-α （TNF-α） and intercellular adhesion molecule-1 （ICAM-1） were measured using RT-PCR. Results The gene expression of IL-1β, TNF-α, ICAM-1 was low in sham group. The mRNA expression of IL-1β was significantly increased and reached the peak after 6h,but began a decrease after 12h and kept the low level at 24h and 48h. The mRNA expression of TNF-α gradually increased after 6 h,and reached the highest levels at 24h, then began to decrease at 48h. The mRNA expression of ICAM-1 gradually increased following cerebral ischemia, and reached the peak at 24h and 48h. Conclusion Our results demonstrated that cerebral ischemia reperfusion could induce the up- regulation of IL-1β,TNF-α and ICAM-1 in a time-dependent way.